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Figure 9 | Respiratory Research

Figure 9

From: Sildenafil attenuates pulmonary inflammation and fibrin deposition, mortality and right ventricular hypertrophy in neonatal hyperoxic lung injury

Figure 9

Paraffin lung sections stained with monoclonal anti-ASMA antibody (panels A-F) and paraffin heart sections stained with HE (panels H-M) after hyperoxic injury for 9 days (panels A-C and H-J) and subsequent recovery in room air for 9 days (panels D-F and K-M) of room-air (RA, panels A and H), O 2 -exposed (panels B and I) and age-matched pups treated with sildenafil (100 mg/kg/day) under hyperoxia (panels C and J), and of RA (panels D and K), O 2 -exposed (panels E and L) and age-matched O 2 -exposed pups treated with sildenafil (100 mg/kg/day, panels F and M) after recovery. Pictures were taken at a 1000× magnification (panels A-F) or at a 40× magnification (panels H-M). Quantification of pulmonary arteriolar medial wall thickness (panel G) and right ventricular hypertrophy (RV/LV wall thickness ratio, panel N) after hyperoxic lung injury for 9 days (Hyp in panels G and N) and after recovery in room air for 9 days (Hyp + Rec in panels G and N) in room air-exposed (white bars), O2-exposed (black bars) and O2-exposed pups treated with 100 mg/kg/day sildenafil (Sil100, gray bars). LV = left ventricle and RV = right ventricle. *p < 0.05, **p < 0.01 and ***p < 0.001 versus age-matched O2-exposed controls. ΔΔ p < 0.01 and ΔΔΔ p < 0.001 versus room air-exposed controls.

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