Figure 8
Paraffin lung sections stained with polyclonal anti-vWF antibody (panels A-F) after hyperoxic injury for 9 days (panels A-C) and subsequent recovery in room air for 9 days (panels D-F) of room-air (RA, panel A), O 2 -exposed (panel B) and age-matched pups treated with sildenafil (100 mg/kg/day) under hyperoxia (panel C), and of RA (panel D), O 2 -exposed (panel E) and age-matched O 2 -exposed pups treated with sildenafil (100 mg/kg/day, panel F) after recovery. Pictures were taken at a 200× magnification. Quantification of pulmonary vessel density (panel G) and mean linear intercept (panel H) after hyperoxic lung injury for 9 days (Hyp in panels G and H) and after recovery in room air for 9 days (Hyp + Rec in panels G and H) in room air-exposed (white bars), O2-exposed (black bars) and O2-exposed pups treated with 100 mg/kg/day sildenafil (Sil100, gray bars). The enlargements shown in the lower left parts of panels A-F are indicated in the boxed areas. *p < 0.05 and ***p < 0.001 versus age-matched O2-exposed controls.ΔΔΔ p < 0.001 versus room air-exposed controls. δδδ p < 0.001 versus own treatment controls in hyperoxia period (hyp).