Figure 6
Bar graphs depicting 3 H-thymidine incorporation into ATII cells (A) and cell number (B) following treatment for 48 hours with VEGF 5 ng/ml, sflt 10 ng/ml and combination confirming a significant proliferation with VEGF 5 ng/ml (#p < 0.001 vs serum control). The presence of sflt not only inhibited the proliferative effect of VEGF but also reduced proliferation below that of serum controls (*p < 0.05), suggesting an autocrine effect. Cell number also increased significantly with 5 ng/ml VEGF165 (**p < 0.01) compared to serum control. The addition of sflt alone and in combination with VEGF165 showed no significant difference compared to serum control (p > 0.05) but did significantly inhibit VEGF165-induced proliferation (p < 0.001 compared to 5 ng/ml VEGF165).