Figure 2

A): Representative RT-PCR gel for human VEGF isoforms in normal, early and later ARDS, lanes as follows: (1) early ARDS, (2) later ARDS, (3) negative control, (4,5,6) sequenced positive controls (VEGF ₁₂₁ , VEGF ₁₆₅ and VEGF ₁₈₉ respectively), (7) 100 kb ladder (bottom marker denotes 100 bp, top marker 300 bp). B-E): Semiquantitative densitometry of (B) VEGF₁₈₉, (C) VEGF₁₆₅, (D) VEGF₁₂₁ relative to β-microglobulin (B₂M) in normal, early and later ARDS (*p < 0.05 late versus early ARDS), (E) ratio of soluble (VEGF₁₂₁, VEGF₁₆₅) to cell-associated (VEGF₁₈₉) isoforms (*p < 0.05 early versus normal and later ARDS). Data are plotted as means with bars denoting standard errors (ANOVA with post-hoc Bonferroni) for B-E).