Figure 1

A) Immunocytochemistry of human whole lung tissue (× 40) showing (A) isotypic control (from later ARDS), (B) normal controls, (C) early ARDS, (D) later ARDS (magnification × 40). Immunostaining shows positive VEGF expression in alveolar epithelium (AE), alveolar macrophages (AM) and (to a lesser extent) vascular endothelium (VE). Significant increase in staining noted in ARDS, especially later ARDS. Staining was assessed semiquantitatively using Histometrix software analysis. B) Histometric analysis of human VEGF in healthy human lung, early and later ARDS. *P < 0.001 in later ARDS versus early ARDS and normal, (ANOVA with post-hoc Bonferroni). Data are normal and plotted as mean and standard error.