Figure 6

Cav1 and SREBP expression in DC from WT and CF mice infected with P. aeruginosa. DC from WT and CF mice were infected in vitro with P. aeruginosa for 4 h, and uninfected cells served as the control (Co). A. Western analysis of Cav1 and corresponding GAPDH. B. Western analysis of SREBP and corresponding GAPDH. C. Luciferase assay of SRE transcriptional activity of CF and WT DC infected with P. aeruginosa. DC were infected with AdZ-SRE-luc for 48 h, and then infected with P. aeruginosa for 4 h. DC were harvested for luciferase assay and β-galactosidase assay. Data is shown luciferase activity (RLU) normalized to β-galactosidase. Shown is the mean ± SEM of three of independent samples. **denotes p < 0.01.