Table 1 Primers used for quantitative real-time PCR
From: Early biomarkers and potential mediators of ventilation-induced lung injury in very preterm lambs
Gene | GenBank Accession # | Nucleotides amplified | Upstream primer 5'-3' | Downstream primer 5'-3' |
|---|---|---|---|---|
EGR1 | DQ239634 | 444–532 | AGGGTCACTGTGGAAGGTC | GCAGCTGAAGTCAAAGGAA |
CTGF | DQ239672 | 407–469 | TATAGCTCCAGCGACAGCTC | ACGAACTTGACTCAGCCTCA |
CYR61 | DQ239628 | 286–354 | ATCGTCCAAACAACTTCGTG | GGTAACGCGTGTGGAGATAC |
IL-1β | NM_001009465 | 353–473 | CGATGAGCTTCTGTGTGATG | CTGTGAGAGGAGGTGGAGAG |
IL-6 | NM_001009392 | 598–705 | CGCAAAGGTTATCATCATCC | CCCAGGAACTACCACAATCA |
IL-8 | NM_001009401 | 438–520 | CCTCAGTAAAGATGCCAATGA | TGACAACCCTACACCAGACC |
18S | X01117 | 1495–1673 | GTCTGTGATGCCCTTAGATGTC | AAGCTTATGACCCGCACTTAC |