Early Growth Response Protein 1 increased with stretch injury and ventilation. (A) Control animals show minimal staining around blood vessels. (B, D, E) The injurious ventilation increased Egr-1 protein surrounding airways, and these increases were not affected by Dex or cortisol. (C) The betamethasone group (Beta) had moderate staining around airways. (F) Semi-quantitative analysis of positive cells per high powered field demonstrated decreased staining in Beta group compared to injurious ventilation. * p < 0.05 vs control, t p < 0.05 vs Injury group.