NFAT inhibitor, VIVIT inhibits hypoxia-induced TRPC1 mRNA up-regulation. Human PASMC were cultured with SMBM (2% FBS) under nomoxia or hypoxia (3% O2) in the presence of sildenafil (100 nM), SKF96365 (7.5 μM) or VIVIT (4 μM) respectively for 72 h. A: PCR amplified products are displayed for TRPC1 (372 bp) and β-actin (539 bp). B: Datanormalized to the amount of β-actin are expressed as mean ± SEM. n = 11, #
P < 0.05 vs. normoxia, *P < 0.05 vs. hypoxia, **P < 0.01 vs. hypoxia.