Figure 4

Inhibitory effect of sildenafil on hypoxia-induced TRPC1 up-regulation. Human PASMC were cultured with SMBM (2% FBS) under normoxia or hypoxia in the presence or absence of sildenafil (100 nM) for 72 h. A: RT-PCR results. a: PCR amplified products are displayed for TRPC1(372 bp) and β-actin (539 bp). b: Data normalized to the amount of β-actin are expressed as mean ± SEM. n = 9, #P < 0.05 vs. normoxia, *P < 0.05 vs. hypoxia. B: Western Blotting results. a: Western boltting results are displayed for TRPC1 (87 kDa) and β-actin (42 kDa). b: Data normalized to the amount of β-actin are expressed as means ± SEM. n = 28, ^# P < 0.05 vs. normoxia, *P < 0.05 vs. hypoxia.