Figure 1

Hypoxia-induced human PASMC proliferation and its dependence on SOC. Human PASMC were cultured with SMBM (2% FBS) in normoxia or hypoxia for different time. A: Phase contrast image of cultured human PASMC (×200). B: Cell viability was determined by MTT. n = 3, **P < 0.01, ΔP < 0.05. C: Cell viability was determined before (Basal) and after 72 h incubation under normoxia and hypoxia without (Control) or with different agents: sildenafil (Sil 100 nM), nifedipine (1 μM), GdCl₃ (1 μM), SKF96365 (7.5 μM), Cyclosporin A (0.03 mg/mL) and EDTA (2 mM), respectively. n = 3, ^### P < 0.001 vs. hypoxia basal, * P < 0.05 vs. hypoxia control, ***P < 0.001 vs. hypoxia control.