Table 1 Ussing chamber solutions (in mM)
From: Secondhand smoke inhibits both Cl- and K+ conductances in normal human bronchial epithelial cells
| Â | Normal buffer | High Cl- | Low Cl- | High K+ | Low K+ |
|---|---|---|---|---|---|
NaCl | 120 | 145 | Â | 25 | 25 |
NaHCO3 - | 25 | Â | Â | Â | Â |
K2HPO4 | 0.8 | 0.8 | 0.8 | 0.8 | 0.8 |
KH2PO4 | 3.3 | 3.3 | 3.3 | 3.3 | 3.3 |
CaCl2 | 1.2 | 1.2 | 1.2 | 1.2 | 1.2 |
MgCl2 | 1.2 | 1.2 | 1.2 | 1.2 | 1.2 |
Na gluconate | Â | Â | 145 | Â | 120 |
K gluconate | Â | Â | Â | 120 | Â |
Glucose | 10 | 10 | 10 | 10 | 10 |
HEPES | Â | 10 | 10 | 10 | 10 |
Gas lift | 95% O2/5% CO2 | Air | Air | Air | Air |
pH | Â | 7.4 | 7.4 | 7.4 | 7.4 |