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Figure 1 | Respiratory Research

Figure 1

From: Secondhand smoke inhibits both Cl- and K+ conductances in normal human bronchial epithelial cells

Figure 1

Smoke Exposure System and quantification of exposure. A) Secondhand smoke (SHS) was generated as described in the text. B) CO levels in the exposure chamber can be varied by diluting a single cigarette's smoke with fresh air (Dilute) or by smoking more than one cigarette simultaneously as indicated by the number of cigarettes shown above the tracing. Temperature and humidity (C) and carbon monoxide (D) are held constant after an initial equilibration period. E) Nicotine can be recovered from the mucosal surface of cells in the exposure chamber but not from sham-exposed cells. F) Sham and SHS-exposed (SHS) HBECs released LDH into the serosal exposure buffer to the same degree, and both released significantly less than cells permeabilized with 0.1% Triton-X100 as a positive control (PC) (*** p < 0.001 compared to both sham and SHS by one-way ANOVA with Tukey's post-tests, n = 4 inserts per condition). Exposure buffer not exposed to cells is shown as a negative control (NC) to demonstrate background.

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