Figure 5

Series of images representing a typical mature alveolus from a 12-day old PDGF-Rα-GFP mouse. A representative 3 μm interval confocal z-stack of a single alveolus from the entry ring (A) to the base (E). The lung tissue was stained using Cy3-α-smooth muscle actin antibody (red) and the lipophilic carbocyanine dye, DiD (blue) for staining cell plasma membranes. The long white arrow shows a "high-expressing-PDGF-Rα " (high intensity of GFP) nucleus, and the white arrowhead shows a "low-expressing-PDGF-Rα-GFP" nucleus. The scale bar shown in (E) is 20 μm.