Table 1 Antibodies used to characterise the epithelial response to TS in SH rats.
Antigen | Source | Catalogue No. | Clone and Isotype | Conc, μg/ml | Antigen Retrieval | Detection System |
|---|---|---|---|---|---|---|
Caspase 3, activated | Cell Signalling | 9661 | Rabbit IgG | 1.0 | MW, citrate | StreptABC HRP, DAB |
CC10 | Cell Signalling | 07-623 | Rabbit IgG | 1.0 | MW, VUF | StreptABC HRP, DAB |
CD31 | Serotec | MCA1334G | TLD-3A12, IgG1 | 6.67 | BA | TSA kit, HRP, DAB |
Ki67 | Vector | VP-K452 | MM1, IgG1 | 0.5 | PC, VUF | TSA kit, HRP, DAB |
CK13 | Chemicon | CBL176 | Ks13.1, IgG1 | 0.5 | PC, VUF | Envision HRP, DAB |
Pan CK (4,5,6,8,10,13,18) | Chemicon | Mab 1636 | C11, IgG1 | 5.0 | MW, VUF | StreptABC HRP, DAB |
p40 | Calbiochem | PC373 | Rabbit IgG | 1/2000 diln | MW, citrate | StreptABC HRP, DAB |
p63 | Pharmingen | 559951 | 4A4, IgG1 | 5 | None | StreptABC HRP, DAB |
Phospho-p38 | Santa Cruz | 17852 | Rabbit IgG | 1 | None | TSA kit, HRP, DAB |
Surfactant D | Abcam | AB15687 | SPDE, IgG2b | 5 | PC, VUF | TSA kit, HRP, DAB |