Disparate oxidant gene expression of airway epithelium compared to alveolar macrophages in smokers

Respiratory Research

Table 5 Pathway Analysis of Smoking Responsive Genes in Small Airway Epithelium and Alveolar Macrophages¹

Small airway epithelium			Alveolar macrophages
Functional pathway	Ratio ²	p value	Functional pathway	Ratio	p value
Xenobiotic metabolism	0.05	< 0.001	IL-10 signaling	0.07	< 0.001
Xenobiotic signaling	0.05	< 0.001	Peroxisome proliferator activator pathway/retinoic acid × receptor activation	0.04	< 0.001
Arachidonic acid metabolism	0.04	< 0.001	Liver × receptor activation pathway	0.06	< 0.001
Pentose and glucuronate interconversions	0.03	< 0.001	Acute phase signaling pathway	0.04	< 0.001
Glutathione metabolism	0.05	< 0.001	Hepatic cholestasis	0.04	< 0.001

¹ Functional pathway analysis was carried out using Ingenuity Pathway Analysis http://www.ingenuity.com on all smoking responsive (fold-change healthy smokers compared to healthy nonsmokers >1.5, p < 0.05 following Benjamini-Hochberg multiple test correction) genes in small airway epithelium and alveolar macrophages from the same healthy nonsmokers (n = 19) and healthy smokers (n = 30). Canonical pathways were selected on the basis of significance and ratio
² Ratio refers to the number of pathway genes in the smoking responsive dataset compared to the total number of genes in the curated pathway.

ISSN: 1465-993X