Table 1 Real-time PCR primers and probes
From: Generation of lung epithelial-like tissue from human embryonic stem cells
Gene | Primers (5' to 3') | Probe | Amplicon length |
|---|---|---|---|
GAPDH | ATG-GAA-ATC-CCA-TCA-CCA-TCT-T | CAG-GAG-CGA-GAT-CC | 57 |
CGC-CCC-ACT-TGA-TTT-TGG | |||
UBC | CGC-AGC-CGG-GAT-TTG | TCG-CAG-TTC-TTG-TTT-GTG | 58 |
TCA-AGT-GAC-GAT-CAC-AGC-GA | |||
FOXJ1 | GAT-CAC-GGA-CAA-CTT-CTG-CTA-CTT-C | CCA-CGC-AGA-TCC-CA | 74 |
GAG-ACA-GGT-TGT-GGC-GGA-TT | |||
SP-C | GTC-TCC-ACA-TGA-GCC-AGA-AAC-A | ACG-GAG-ATG-GTT-CTG-G | 57 |
GCG-CCC-CAA-TGC-TCA-TC | |||
CC16 | CCC-TGG-TCA-CAC-TGG-CTC-TC | TGC-AGC-TCC-GCT-TC | 61 |
TGA-AAG-CTC-GGG-CAG-ATC-TC | |||
Â-Tubulin IV | GAT-CTT-TCG-GCC-GGA-CAA | TTG-GCC-AAT-CCG-G | 65 |
GCC-CCT-TTG-CCC-AGT-TG | |||
NKX2.1 | CAC-ACG-ACT-CCG-TTC-TCA-GTG-T | TGA-CAT-CTT-GAG-TCC-CCT-G | 66 |
GCC-CAC-TTT-CTT-GTA-GCT-TTC-C | |||
POU5F1 | GGA-CAC-CTG-GTC-TGC-GAT-TT | GCC-TTC-TCG-CCC-CC | 54 |
CAT-CAC-CTC-CAC-CAC-CTG-G | |||
Aq5 | Hs00387048_m1* | 68 | |
Vimentin | Hs00185584_m1* | 73 | |
Nanog | Hs02387400_g1* | 109 |