Figure 9
From: Generation of lung epithelial-like tissue from human embryonic stem cells
Immunofluorescence staining for three stem cell markers: TRA1-60 (A,D), TRA1-81 (B,E) and SSEA4 (C,F). 4 μm thick sections of hESC grown with the ALI differentiation protocol for 20 days (D-F) were compared to sections of undifferentiated hESC control samples (A-C). Undifferentiated hESC showed extensive staining for all three markers. After 20 days of differentiation in ALI conditions only sporadic areas of staining could be observed. Representative results of VUB07 are shown. Original magnification: 200× (A, D-F) and 400× (B-C).