Figure 6
From: Generation of lung epithelial-like tissue from human embryonic stem cells
Immunostaining of SP-C. hESC were grown with the ALI differentiation protocol for 20 days (B-D). Images of two different cell lines (VUB03_DM1 (B-C) and VUB04_CF (D) were scanned by confocal microscopy after immunostaining with SP-C antibody (green) and nuclear DAPI staining (blue). Figure 6A shows immunostaining of SP-C in fetal lung (20 weeks old). Original magnification 200× (A,B) and 600× (C-D).