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Figure 5 | Respiratory Research

Figure 5

From: Generation of lung epithelial-like tissue from human embryonic stem cells

Figure 5

Immunostaining of CC16. Representative image of CC16 immunostaining (green) in combination with nuclear DAPI staining (blue) in undifferentiated hESC control samples (A), in samples cultured with the ALI differentiation protocol (VUB03_DM1)(B) and in fetal lung (20 weeks old)(C). Staining was found in the cytoplasm of the cells lining the bronchiolar epithelium (C) and in the cells lining the cavities (B) of the tissue formed by ALI culture. No staining was seen in undifferentiated hESC controls (A). Original magnification 200× (A) and 400× (B-C) These experiments were also carried out with VUB04_CF and with VUB09_FSHD hESC lines and showed similar results (data not shown).

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