Figure 3
From: Generation of lung epithelial-like tissue from human embryonic stem cells
Relative quantification of NANOG and POU5F1 mRNA levels. Relative quantification of NANOG and POU5F1 mRNA levels in samples from human embryonic stem cells (hESC) cultured on porous membranes with the ALI differentiation protocol (ALI), compared to the NO ALI control samples. Samples were collected on days 5, 10, 15, and 20, and data are represented as means ± SD. NANOG and POU5F1 mRNA levels decreased over time in both conditions and no statistical difference could be observed. Two endogenous controls were used (GAPDH and Ubiquitin C). Results of VUB04_CF are shown.