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Figure 2 | Respiratory Research

Figure 2

From: Generation of lung epithelial-like tissue from human embryonic stem cells

Figure 2

Quantitative RT-PCR analysis of Aquaporin 5 , FOXJ1, β-tubulin IV and SP-C mRNA levels at day 20. Aquaporin 5, FOXJ1, β-tubulin IV and SP-C mRNA levels were measured in samples from hESC cultured on porous membranes with the ALI differentiation protocol and in NO ALI control samples. After 20 days of ALI culture Aquaporin 5, FOXJ1 and β-tubulin IV are all significantly upregulated (p < 0.05) as is SP-C compared to NO ALI control samples. Note that the y-axis is on a logarithmic scale. The samples from hESC spontaneous differentiation control samples were considered as a reference and data represent the mean ± SD. Experiments were performed at least three times and at least two different samples were taken for each measurement to provide biological replicates. Data are shown for VUB07. These experiments were also carried out with VUB03_DM1, VUB04_CF and VUB14 and showed similar results (data not shown). Two endogenous controls were used (GAPDH and Ubiquitin C).

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