Figure 4

A representative immunofluorescence staining of type I-like alveolar epithelial cells after (A, D) mock, (B, E) influenza H1N1 and (C, F) H5N1 virus infection and (G) chart with percentage of infection. Virus matrix protein and nucleoprotein were stained green by FITC-conjugated mouse antibody. The immunofluoresecent staining of the type I-like alveolar epithelial cells after apical (A-C) and basolateral (D-F) infection at 24 h post infection respectively. (G) Bar chart shows the mean percentage of infection and error bar represent the standard derivation, dark bar represents apical infection and open bar represents basolateral infection. Single and double asterisk indicates statistically significant difference with p < 0.05 and p < 0.01 respectively.