Table 1 Real-time PCR primer and probe sequences
Target | Sense primer (5' ⇒ 3') | Antisense primer (5' ⇒ 3') | Probe (5'FAM ⇒ 3'BHQ) |
|---|---|---|---|
E-cadherin | CCACCAAAGTCACGCTGAATAC | GGAGTTGGGAAATGTGAGCAA | CCATCAGGCCTCCGTTTCTGG |
α-SMA | CTGGCATCGTGCTGGACTCT | GATCTCGGCCAGCCAGATC | ATGCCTTGCCCCATGCCATCA |
Tenascin C | CAGAAGCCGAACCGGAAGTT | TTCATCAGCTGTCCAGGACAGA | TGCCACCCCAGACGGTTTCC |
Fibronectin-EDA | GAGCTATTCCCTGCACCTGATG | CGTGCAAGGCAACCACACT | TGCAAGGCCTCAGACCGGGTTC |
Collagen I | CCTCAAGGGCTCCAAC | GGTTTTGTATTCAATCACTGTCTTGC | ATGGCTGCACGAGTCACACCGGA |
Vimentin | GGAAGAGAACTTTGCCGTTGAA | GTGACGAGCCATTTCCTCCTT | CCAAGACACTATTGGCCGCCTG |
β-actin | TGCGTGACATTAAGGAGAAG | GTCAGGCAGCTCGTAGCTCT | CACGGCTGCTTCCAGCTCCTC |
2-microglobulin | AGCGTCTCCAAAGATTCAG | AGACACATAGCAATTCAGGA | ACTCACGTCATCCAGCAGAGAATGG |