Figure 9
Corticosteroids do not completely prevent TGFβ 1 induced EMT. BEAS-2B cells were pretreated with dexamethasone (dex, 10-7 M) or budesonide (bud, 10-8 M) for 16 hours followed by subsequent stimulation with TGFβ1 (1 ng/ml) for one or 3 days. mRNA expression levels were assessed by quantitative realtime PCR for E-cadherin (1 day) and αSMA, collagen I, fibronectin-EDA and tenascin C after 3 days. Results are representative of 3 separate experiments, each in triplicate. (*p < 0.05 compared to non-TGFβ1 stimulated controls by Mann-Whitney U test).