Figure 8
IL-1β reduce the expression of E-cadherin and enhances the effects of TGFβ on Tenascin C expression. BEAS-2B cells were stimulated with TGFβ1 (0.1 ng/ml) or IL-1β (1 ng/ml) or both for 3 days in triplicate. Total RNA was isolated and assessed in triplicate for the expression of E-cadherin and Tenascin C by means of quantitative real-time PCR. Expression levels were normalized to the housekeeping gene β-actin and calculated as mean level of induction in comparison to control untreated cells. Results show mean ± standard error of 3 separate experiments, each in triplicate (* p < 0.0001 compared to control; ‡ p < 0.02 compared to TGFβ1 treated; §p < 0.0005 compared to IL-β treated; all by Mann-Whitney U test).