Figure 6
TGFβ 1 increases the mRNA of EMT-marker proteins in primary human bronchial epithelial cells (NHBE). A: NHBE cells were stimulated with TGFβ1 (2 ng/ml) or complete medium alone (control) for 3 days in triplicate. The levels of αSMA and E-cadherin mRNA were analyzed by quantitative real-time PCR. Expression levels were normalized to the housekeeping gene 2-microglobulin and calculated as fold induction in comparison to control. Results are representative of experiments performed with 2 different donors. (*p < 0.05 compared to control by Mann-Whitney U test) B: Cell lysates from NHBE cells, stimulated for the indicated time with TGFβ1 (2 ng/ml) or complete medium, were immunoblotted with for E-cadherin or αSMA. Blots were reprobed for β-actin as loading control.