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Figure 5 | Respiratory Research

Figure 5

From: TGF-β1 induced epithelial to mesenchymal transition (EMT) in human bronchial epithelial cells is enhanced by IL-1β but not abrogated by corticosteroids

Figure 5

TGFβ 1 increases migration and invasion of BEAS-2B cells. A: BEAS-2B cells pre-stimulated with TGFβ1 (5 ng/ml) or complete medium alone for 3 days, followed scratch wounding with a 200 ul pipette tip at ~90% confluence. Pictures of the same area were taken under bright field illumination immediately after wounding (0 h) as well as 24 h and 48 h later. B: BEAS-2B cells were pre-stimulated with TGFβ1 (5 ng/ml) or complete medium alone for 4 days, seeded on Matrigel coated inserts in complete medium without the addition of TGFβ1 and incubated for 24 h. Epithelial cells, which had migrated through the inserts were counted under light microscope at 10× magnification. The number of invading cells after TGFβ1 treatment were normalized to the number of invading control untreated cells, which were set as 100%. Data are averaged from three independent experiment each performed triplicate. (*p < 0.0001 compared to control by unpaired Wilcoxon-Mann-Whitney Rank Sum Test). C: Conditioned media of BEAS-2B cells stimulated with TGFβ1 (5 ng/ml) or complete medium alone were subject to gelatin-zymography. Experiments were conducted three times with similar results.

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