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Figure 4 | Respiratory Research

Figure 4

From: TGF-β1 induced epithelial to mesenchymal transition (EMT) in human bronchial epithelial cells is enhanced by IL-1β but not abrogated by corticosteroids

Figure 4

TGFβ 1 increases the expression of connective tissue proteins in BEAS-2B cells. A: BEAS-2B cells were stimulated with TGFβ1 (5 ng/ml) or complete medium alone (control) and the levels of fibronectin-EDA, collagen I and tenascin C mRNA were analyzed by quantitative real-time PCR as described in Fig 2A. (*p < 0.05 by Kruskal-Wallis one-way ANOVA) B: Cell lysates from BEAS-2B cells, stimulated for the indicated time with TGFβ1 (5 ng/ml) or complete medium, were immunoblotted for fibronectin as described in Methods. Blots were stripped and rehybridized with an antibody to β-actin.

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