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Figure 3 | Respiratory Research

Figure 3

From: TGF-β1 induced epithelial to mesenchymal transition (EMT) in human bronchial epithelial cells is enhanced by IL-1β but not abrogated by corticosteroids

Figure 3

Changes of E-cadherin and αSMA protein levels in BEAS-2B cells upon TGFβ 1 treatment. A: Cell lysates from BEAS-2B cells, stimulated for the indicated time with TGFβ1 (5 ng/ml) or complete medium, were immunoblotted for E-cadherin or αSMA as described in Methods. Blots were stripped and rehybridized with an antibody to β-actin. B: Immunofluorescent staining for E-cadherin in BEAS-2B cells stimulated with TGFβ1 (5 ng/ml) for 4 days or complete medium alone. The left panel shows only the E-cadherin fluorescence (pseudocolor green) and the right panel the overlay with the DAPI fluorescence (pseudocolor blue). Images were captured at a magnification of 20×. Results are representative of 3 separate experiments.

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