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Figure 2 | Respiratory Research

Figure 2

From: TGF-β1 induced epithelial to mesenchymal transition (EMT) in human bronchial epithelial cells is enhanced by IL-1β but not abrogated by corticosteroids

Figure 2

Expression changes of E-cadherin and αSMA in BEAS-2B cells upon TGFβ 1 treatment. BEAS-2B cells were stimulated with TGFβ1 or complete medium alone (control) in triplicate for the indicated time and doses. Total RNA was isolated and assessed in triplicate for the expression of E-cadherin, α SMA and β-actin by means of quantitative real-time PCR. Expression levels were normalized to the housekeeping gene β-actin and calculated as mean level of induction in comparison to control untreated cells. A: Time course: Beas-2B cells were stimulated with 5 ng/ml TGFβ1 from one to 5 days. (*p < 0.01 by Kruskal-Wallis one-way ANOVA). B: Dose response: Beas-2B cells were treated with TGFβ1 from 0.001 ng to 10 ng/ml for 5 days (*p < 0.05 compared to control by Mann-Whitney U test).

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Respiratory Research

ISSN: 1465-993X

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