Upregulation of pirin expression by chronic cigarette smoking is associated with bronchial epithelial cell apoptosis
© Gelbman et al. 2007
Received: 09 May 2006
Accepted: 08 February 2007
Published: 08 February 2007
Cigarette smoke disrupts the protective barrier established by the airway epithelium through direct damage to the epithelial cells, leading to cell death. Since the morphology of the airway epithelium of smokers does not typically demonstrate necrosis, the most likely mechanism for epithelial cell death in response to cigarette smoke is apoptosis. We hypothesized that cigarette smoke directly up-regulates expression of apoptotic genes, which could play a role in airway epithelial apoptosis.
Microarray analysis of airway epithelium obtained by bronchoscopy on matched cohorts of 13 phenotypically normal smokers and 9 non-smokers was used to identify specific genes modulated by smoking that were associated with apoptosis. Among the up-regulated apoptotic genes was pirin (3.1-fold, p < 0.002), an iron-binding nuclear protein and transcription cofactor. In vitro studies using human bronchial cells exposed to cigarette smoke extract (CSE) and an adenovirus vector encoding the pirin cDNA (AdPirin) were performed to test the direct effect of cigarette smoke on pirin expression and the effect of pirin expression on apoptosis.
Quantitative TaqMan RT-PCR confirmed a 2-fold increase in pirin expression in the airway epithelium of smokers compared to non-smokers (p < 0.02). CSE applied to primary human bronchial epithelial cell cultures demonstrated that pirin mRNA levels increase in a time-and concentration-dependent manner (p < 0.03, all conditions compared to controls).
Overexpression of pirin, using the vector AdPirin, in human bronchial epithelial cells was associated with an increase in the number of apoptotic cells assessed by both TUNEL assay (5-fold, p < 0.01) and ELISA for cytoplasmic nucleosomes (19.3-fold, p < 0.01) compared to control adenovirus vector.
These observations suggest that up-regulation of pirin may represent one mechanism by which cigarette smoke induces apoptosis in the airway epithelium, an observation that has implications for the pathogenesis of cigarette smoke-induced diseases.
Although the airway epithelium has several defense mechanisms that respond to the stress imposed by cigarette smoke, in some individuals these defenses are exaggerated, resulting in chronic inflammation, and eventually, chronic bronchitis [1–5]. One of the earliest effects of cigarette smoke on the airway epithelium is to disrupt the protective barrier that is normally mediated by tight junctions between epithelial cells, resulting in increased permeability across the epithelium [6–8]. The disruption of the epithelial barrier is associated with an innate immune response, typified by the migration of inflammatory cells into the epithelial layer [3, 4]. If this inflammatory response, becomes chronic, it eventuates into the airway pathology and dysfunction associated with chronic bronchitis .
The mechanism by which the bronchial epithelium becomes disrupted by cigarette smoke is not fully understood, but evidence suggests that damage to the epithelial cell per se is more important than dysfunction of the junctions between epithelial cells [10, 11]. Since the morphology of the airway epithelium of cigarette smokers does not typically demonstrate epithelial necrosis, the most likely mechanism for epithelial cell death in response to cigarette smoke is apoptosis . Consistent with this concept, in vitro studies have shown that exposure to cigarette smoke can initiate apoptosis in fibroblasts, macrophages and alveolar epithelial cell lines [13–15], and apoptosis of airway epithelial cells has been observed in experimental animals in response to cigarette smoke [16, 17], but in vitro studies of bronchial epithelial cells exposed to cigarette smoke have yielded conflicting results [18–20].
In the context of these considerations, we hypothesized that cigarette smoke modulates the expression of genes in the airway epithelium that initiate the cells to undergo apoptosis. To assess this hypothesis, gene expression profiling was used to determine if cigarette smoking in humans is associated with the up-regulation of pro-apoptotic genes (or the down-regulation of anti-apoptotic genes) in the bronchial epithelium. To obtain the relevant biologic samples, we used fiberoptic bronchoscopy with airway brushing to sample the bronchial epithelium from smokers and non-smokers. To avoid the confounding effects of already established lung disease, airway epithelium was sampled from phenotypically normal ~20 pack-yr smokers. The genes that were up-regulated in the smokers were functionally annotated using public databases according to their potential role in apoptosis. Pirin, a transcription cofactor that has been shown in eukaryotic cells to be induced during apoptosis , was selected for further study because it had the highest fold change in expression level in association with smoking. In vitro experiments in which primary and transformed human airway epithelial cell lines were exposed to cigarette smoke extract confirmed that cigarette smoke per se up-regulated pirin mRNA levels. Using an adenovirus gene transfer vector that constitutively expressed pirin, studies in a bronchial epithelial cell line showed that pirin up-regulation was associated with apoptosis. Together, the data suggests that cigarette smoking up-regulates pirin expression in the bronchial epithelium, with an associated increase in apoptosis, thus identifying at least one signaling mechanism associated with the disruption of the airway epithelial barrier in cigarette smokers.
Healthy non-smokers and healthy chronic smokers were recruited using local print advertisements. The study individuals are part of an ongoing project to assess gene expression in the human airway epithelium in regard to the chronic airway disorders associated with cigarette smoking [22–24]. The study was approved by the Weill Cornell Medical College Institutional Review Board and written informed consent was obtained from each individual before enrollment in the study. The smokers had an approximate smoking history of 20 pack-yr and were in otherwise good health, with no evidence of respiratory tract infection, chronic bronchitis or lung cancer. Each individual had to complete an initial screening evaluation, which included a history of smoking habits, respiratory tract symptoms, and prior illnesses, a complete physical exam, chest radiograph, and pulmonary function tests. Routine screening blood and urine studies were performed, including urinary levels of nicotine and its derivative cotinine, and serum levels of carboxyhemoglobin to verify reported levels of smoking.
Collection of Airway Epithelial Cells
All individuals who met the inclusion and exclusion criteria underwent fiberoptic bronchoscopy with brushing of the 3rd to 4th order bronchi as previously described . Smokers were instructed not to smoke the evening before undergoing bronchoscopy. A 1 mm disposable brush (Wiltek Medical, Winston-Salem, NC) advanced through the working channel of the bronchoscope was used to collect the airway epithelial cells by gently gliding the brush back and forth on the airway epithelium 5 to10 times in 10 different locations in the third branching of the bronchi in the right and left lower lobe of each individual. The cells were detached from the brush by flicking it into 5 ml of ice-cold LHC8 medium (GIBCO, Grand Island, NY). An aliquot of 0.5 ml was kept for differential cell count and for cytology; the remainder (4.5 ml) was processed immediately for RNA extraction. Total cell number was determined by counting on a hemocytometer. Differential cell count (epithelial vs inflammatory cells) was assessed on cells prepared by cytocentrifugation (Cytospin 11, Shandon Instruments, Pittsburgh, PA) stained with DiffQuik (Baxter Healthcare, Miami FL).
Preparation of cDNA and Hybridization to Microarray
All analyses were performed using the Affymetrix HuGeneFL chip and associated protocol from Affymetrix (Santa Clara, CA). Total RNA was extracted from brushed cells using TRIzol (Life Technologies, Rockville, MD) followed by RNeasy (Qiagen, Valencia CA) to remove residual DNA, which yielded approximately 2 μg RNA from 106 cells. First strand DNA was synthesized using the T7-(dT)24 primer and converted to double stranded cDNA using Superscript Choice system (Life Technologies). cDNA was purified by phenol chloroform extraction and precipitation, and the size distribution was examined after agarose gel electrophoresis. The cDNA was then used to synthesize biotinylated RNA transcript using the Bioarray HighYield reagents (Enzo, New York, NY). This was purified by RNeasy (Qiagen) and fragmented immediately before use. The labeled cRNA was hybridized to the HuGeneFL GeneChip for 16 hr, and then processed by the fluidics station under the control of Microarray suite software (Affymetrix). The chip was then manually transferred to the scanner for data acquisition.
RNA levels for pirin were measured relative to 18s rRNA by real time quantitative PCR (TaqMan) with fluorescent TaqMan chemistry using the ΔΔCt method (PE Biosystems, Instruction Manual). TaqMan reactions for pirin were optimized and validated to show equal amplication efficacy compared to 18s rRNA using adult human lung RNA (Strategene, La Jolla, CA). Two sets of primers and probes were used, one to measure endogenous RNA (including 3' untranslated end), and one to measure both endogenous and adenovirus-produced pirin mRNA (which spans two exons and would not amplify genomic DNA). The endogenous specific pirin primers were: forward AATGGGTTTGAAAGGGCCA and reverse TCAAGACCTGCTCTTCCGCT, with probe AACCTGGAAATCAAAGATTGGGAACTAGTGGA. The endogenous and adenovirus-produced pirin primers were: forward CACGCTGAGATGCCTTGCT and reverse ACCATCTTCTCTGAGCTCCTCAA with probe CAGCCCATGGCCTACAACTGTGGGTTATA.
Exposure of Primary Human Bronchial Epithelial Cells to Cigarette Smoke Extract
Three separate primary human bronchial epithelial (HBE) cell cultures were isolated from trachea and bronchi of donor lungs and seeded onto collagen-coated semi-permeable membranes (Millipore, Bedford, MA) and grown at the air-liquid interface . The viability of the cells was confirmed before each experiment by measurement of transepithelial resistance .
Cigarette smoke extract (CSE) was prepared using a modification of the method used by Wyatt et al . Four research grade cigarettes (2R4F, University of Kentucky) were bubbled into 50 ml of 1:1 DMEM:Ham F12 medium using a vacuum pump apparatus. The CSE was filtered through a 0.22 μm filter to remove particles and bacteria before use. Solutions of 10% and 100% CSE were prepared from this stock. The solution of CSE (15 μl) was applied to the apical surface of the HBE cells and RNA was isolated from the cultures at 2, 24, and 48 hr after CSE exposure using TRIzol (Life Technologies) followed by RNeasy (Qiagen). Samples were obtained in triplicate for each time point. Pirin RNA levels were measured by TaqMan RT-PCR using the primers and probe described above. Pirin expression levels relative to 18s rRNA were assessed. Each data point was generated from triplicate wells for each of the three separate cell lines.
Assessment of Pirin-induced Apoptosis
To assess the relationship between up-regulation of pirin and the induction of apoptosis, an adenovirus (Ad) gene transfer vector coding for pirin was used to transfer the human pirin cDNA to human bronchial epithelial cells, and pirin expression and apoptosis were assessed over time. The recombinant Ad vectors AdPirin and AdNull used in this study are E1a-, partial E1b-, and partial E3-, based on the Ad5 genome, with the expression cassette in the E1 position [27–29]. The AdPirin expression cassette includes the cytomegalovirus early/intermediate enhancer/promoter (CMV), an artificial splice signal, the human pirin cDNA (obtained from A549 cells), and an SV40 stop/poly (A) signal. The AdNull vector is identical to the AdPirin vector, except that it lacks a cDNA in expression cassette . The vectors were propagated, purified, and stored at -70°C .
AdPirin-induced apoptosis was assessed in the human airway epithelial BEAS-2B cell line . BEAS-2B cells (ATCC, Rockville, Maryland) were grown on lysine coated coverslips in LHC-9 medium (Biosource International), Camarillo, CA) until they were 50 to 60% confluent. The cells were then infected with AdNull and AdPirin at varying concentrations [103 and 104 particle units (pu)].
Two assays were used to assess apoptosis: TdT-mediated dUTP nick end labeling (TUNEL) assay and cytoplasmic nucleosome ELISA. For the TUNEL assay, cells were fixed to the coverslips using 4% paraformaldehyde and then permeabilized with 0.2% Triton X-100 in PBS. Cells were equilibrated with equilibration buffer, nucleotide mix, and rTdT enzyme (Promega, Madison WI) for 60 min and then washed. DAPI nuclear counterstain was applied before cells were mounted onto slides and evaluated under fluorescent microscope. The percentage of apoptotic cells per 10× field were manually counted in 10 fields per slide. For the cytoplasmic nucleosome ELISA assay (Cell Death Detection ELISA, Roche, Indianapolis, IN), BEAS-2B cells were lysed with lysis buffer, centrifuged for 10 min at 200 × g to pellet nuclei. The supernatant (20 μl) was added to the immunoreagent containing anti-histone biotin and anti-DNA horseradish peroxidase (HRP). Sample wells were placed on shaker at 300 rpm for 2 hr, 23°C. 2, 2azino-di[3-ethylbenzthiazolin-sulfonate] (ABTS) solution was added and photometric analysis was measured at 405 nm, subtracted from background 490 nm. For each sample, the fluorescent value was normalized to the internal negative control of the experiment to generate an apoptotic index, which reflects the fold change in the number of apoptotic cells for experimental condition compared to control.
A 32P-labeled pirin specific DNA probe was synthesized using strip EZ labeling kit (Roche, Indianapolis, IN) from a pirin cDNA template amplified from human genomic DNA. RNA electrophoresis was performed in 1% agarose gel followed by transfer to nitrocellulose membrane and UV crosslinking. The membrane was then hybridized with DNA probe and exposed to X ray film for 1 hr.
The microarray data was analyzed using the GeneSpring software (Silicon Genetics, Redwood City, CA). Normalization was carried out sequentially: per microarray sample (dividing the raw data by the 50th percentile for all measurements) and then per gene (dividing the raw data by the median of the expression levels for the given gene in all samples). Data from probe sets representing genes that failed the Affymetrix detection criteria (labeled "Absent" or "Marginal") in all 44 microarrays were eliminated from further analysis. The p value for each gene was calculated comparing the non-smokers with smokers using the Welch t-test with a Benjamini-Hochberg correction for false discovery rate. For the in vitro studies, comparisons between RNA expression levels for pirin and percentage of apoptotic cells were made using the Student's two-tailed t-test.
Apoptosis-relevant Genes Up-regulated in the Airway Epithelium of Smokers1
Smokers/non-smokers (fold up)
Reference relevant to apoptosis
retinoic acid receptor responder 2
prostate differentiation factor/growth differentiation factor 15
insulin-like growth factor binding protein 5
bone morphogenetic protein 7 (osteogenic protein 1)
carcinoembryonic antigen-related cell adhesion molecule 6
S100 calcium-binding protein A10
Up-regulation of Pirin in Cigarette Smoker's Bronchial Epithelium In Vivo
Pirin Gene Expression Following Cigarette Smoke Exposure In Vitro
Induction of Bronchial Epithelial Cell Apoptosis in Association with Up-regulation of Pirin Expression
Cigarette smoking is the major environmental exposure that leads to the pathogenesis of COPD [1–5]. The oxidative stress that cigarette smoke places on the airway epithelium results in a series of predictable morphologic changes over time. Despite our understanding of the pathologic changes that occur in the airways, the molecular mechanisms that direct the response of the airway epithelium to cigarette smoke are only partially understood [1–5]. The present study on pirin emerged from our laboratory's ongoing effort to identify unique cellular pathways that are linked to the development of lung diseases, such as COPD and lung cancer, by using gene expression analysis of the airway epithelium of smokers.
Apoptosis of airway epithelial cells is relevant to the pathogenesis of chronic bronchitis because disruption of epithelial integrity in the central airways appears to be an early event in response to cigarette smoke [6–8, 32]. Based on the hypothesis that there may be apoptosis-related proteins expressed in the epithelial cells of cigarette smokers that have not been identified, we employed the unbiased strategy of assessing gene expression in airway epithelial cells of smokers vs non-smokers for the up-regulation of candidate proteins using microarray technology. Affymetrix gene chip assessment of airway epithelial cells from phenotypically normal ~20 pack-yr smokers compared to normal non-smokers demonstrated the up-regulation of several proteins that have been linked to apoptosis in the airway epithelium of smokers compared to non-smokers. Among the up-regulated proteins that were found to be associated with apoptosis was pirin, a nuclear transcription cofactor that is part of the cupin superfamily [21, 33, 34].
TaqMan real-time RT-PCR independently confirmed the microarray observed changes in pirin mRNA expression. To validate this in vivo observation, an in vitro model was designed to test the effect of cigarette smoke on pirin expression in epithelial cell culture. Using primary cultures of human bronchial epithelial cells, pirin expression was shown to respond to increasing concentrations of cigarette smoke extract relatively acutely, within 24 to 48 hr. A similar increase in pirin RNA expression level was observed in the BEAS-2B cell line when exposed to cigarette smoke extract. The potential for pirin to induce apoptosis in bronchial epithelial cells was assessed by using AdPirin, an adenovirus gene transfer vector expressing the human pirin cDNA, to over-express pirin in cultures of transformed airway epithelial cells. The data demonstrate that pirin over-expression results in significant epithelial cell apoptosis, a possible mechanism that leads to a breakdown of the epithelium in response to cigarette smoke. Although the magnitude of pirin over-expression is greater in the in vitro AdPirin experiments than that observed in vivo, it is important to recognize that the in vivo upregulation of pirin expression occurs after chronic smoking (~20 pack-yr), and this chronic, low level up-regulation over an extended period of time is impossible to emulate in vitro. It is therefore not surprising, that in order to demonstrate an in vitro effect on apoptosis over a much shorter time course (2–3 days), a higher expression of the gene was required. Interestingly, in the experiments where bronchial epithelial cells (BEAS-2B) were exposed to cigarette smoke extract, there was a similar 2 fold increase in pirin expression to 10% CSE and a significant increase in apoptosis, thus paralleling the in vivo observation.
Apoptosis and the Pathogenesis of Chronic Bronchitis
One of the main functions of the airway epithelium is to provide a physical barrier to protect the underlying tissue from the harmful effects of cigarette smoke [1, 2, 4, 5]. The inflammatory response seen in COPD is initiated when the airway epithelium is disrupted by chronic exposure to cigarette smoke . This breach in epithelial integrity triggers the innate immune response which mobilizes neutrophils, eosinophils, macrophages, natural killer cells and mast cells into the subepithelial space [2, 9]. When the stress of cigarette smoke is exerted over years, the persistent inflammation in the central airways leads to the pathologic finding of chronic bronchitis. As the inflammation extends to the small airways (between 4th to 14th generation bronchi), inflammatory exudates can accumulate in the lumen of the bronchi resulting in airflow obstruction .
Epithelial permeability, as measured by 99mTC-DTPA lung clearance in human subjects, has been shown to be increased in chronic smokers compared to non-smokers [7, 32]. Studies using animal models have shown that epithelial cell permeability can increase in response to cigarette smoke with 30 min and 6 hr of exposure [8, 11]. This is followed by an increased number of neutrophils and mast cells in the airway wall 6 hr after cigarette smoke exposure. The mechanism for increased permeability is unclear, but prior studies have shown that damage to the epithelial cell itself, and to a lesser extent, disruption in the tight junctions between epithelial cells are partly responsible [10, 11]. Consistent with the bronchial epithelial cell studies, in vitro cellular studies have shown that alveolar epithelial cell viability decreases after exposure to cigarette smoke in a time and concentration dependent manner .
The mechanism of cell death for the epithelium in response to cigarette smoke may involve some necrosis, but apoptosis is likely the dominant mechanism . Mouse models of smoking induced airway disease have demonstrated that apoptosis can occur in the airways in response to cigarette smoke [16, 17]. Due to the efficient nature of the apoptotic pathway, allowing for rapid clearance of dying cells, it can be technically difficult to assess for apoptosis from in vivo studies of the airway in humans.
Several in vitro studies have shown that apoptosis can occur in response to cigarette smoke extract (CSE) in a variety of cell types, including airway epithelial cells, alveolar macrophages, endothelial cells, alveolar epithelial cells, and fibroblasts [13–15]. In contrast, Wickenden et al  found that CSE induced necrosis of a variety of cell types, while inhibiting the activation of the caspase system and preventing apoptosis. This discrepancy may be explained by the observation that at low concentrations, CSE induces apoptosis, while at higher concentrations, necrosis is induced . Liu et al  reported that CSE caused DNA damage and initiated DNA repair processes, but not apoptosis, in bronchial epithelial (BEAS-2B) cells. The reason their conclusions differ from ours is likely due to differences in the concentration of cigarette smoke extract used. The maximum concentration that Liu  et al used was 10% CSE generated from 1 cigarette bubbled into sterile water. Similarly, in our experiment we did not observe apoptosis in BEAS-2B cells exposed to 10% CSE, but did observe apoptosis in cells exposed to 50% CSE, generated from 4 cigarettes bubbled into LHC-9 media. Taking all of the in vitro studies into consideration, one potential explanation is that at low concentrations, CSE causes DNA damage that is reparable until a threshhold concentration is reached, after which the cell initiates apoptosis. Very high levels of CSE likely cause overt necrosis.
Induction of Apoptosis by Pirin
Pirin was first identified by Wendler et al , where it was found to interact with nuclear factor 1/CCAAT box transcription factor (NF1/CTF1). Pirin was found to be exclusively nuclear, expressed in most tissues and highly conserved among mammals. The N-terminus shares significant homologies with proteins from plants, fungi and prokaryotes.
Pirin was later found to bind to the oncoprotein B-cell lymphoma 3-encoded (Bcl-3), a member of the IκB family. Bcl-3 interacts with the anti-apoptotic protein NF-κB, by binding to the p50 and p52 . Recently, the crystal structure of pirin has been elucidated and was found to have a single Fe2+ ion in the highly conserved N-terminal region . This metal ion may be necessary for its interaction with Bcl-3 and NF-κB. Bcl-3 can either inhibit or enhance NF-κB DNA binding and transcription depending on its binding to other transcription co-factors.
Pirin stabilizes the formation of quaternary complexes between Bcl-3, NF-κB, and its DNA target. While it is not clear exactly how pirin influences the interaction between Bcl-3 and NF-κB, based on our results and others, it seems possible that pirin directs NF-κB DNA binding towards a pro-apoptotic response. Elucidating this mechanism will require further investigation to understanding the signaling that results from pirin expression. This may not be possible through gene expression studies alone, since several apoptotic pathways are induced by mechanisms other than up-regulation of mRNA levels of pro-apoptotic genes. This might explain why there was no observed upregulation of traditional apoptotic or inflammatory pathway genes, e.g., caspases and Bcl-2, in our microarray data obtained from human airway epithelium. The lack of up-regulation of pro-inflammatory genes in the airway epithelium of smokers is also supported by the transcriptome analysis of Spira et al . One possible explanation for this observation is that many of these inflammatory mediators may be released primarily by non-epithelial cells.
Evidence for pirin involvement in apoptosis comes from experiments in tomatoes . When tomato cells in suspension are treated with the topoisomerase inhibitor, campothecin, a potent initiator of cell death, le-pirin, an ortholog to human pirin, mRNA levels are dramatically increased. In the cyanobacterium Synechocystis, pirin orthologs are highly induced under conditions of severe salt stress .
We originally noted pirin to be up-regulated in microarrays of phenotypically normal individuals that smoke , a finding also made by Spira et al , in their study of genes up-regulated in the airway epithelium of smokers. Interestingly, Spira et al  observed that the pirin levels returned to normal for ex-smokers, supporting observation in the present study that pirin is influenced by acute exposure to cigarette smoke.
Our current study suggests that pirin expression in the airway epithelium is in response to the acute oxidative stress imposed by cigarette smoke. Our study also suggests that pirin over-expression in response to cigarette smoke may play a role in epithelial cell apoptosis, and one possible mechanism is by interacting with NF-κB to induce the expression of pro-apoptotic genes, in cells injured by cigarette smoke . Theoretically, apoptosis of the bronchial epithelium in response to smoking is a delicate balancing act. On the one hand, it is probably necessary to prevent DNA damage and subsequent mutagenesis that leads to lung cancer. On the contrary, it can also lead to a loss of epithelial integrity followed by chronic inflammation that results in chronic bronchitis.
We thank J Kaplan and N Mohamed for help in preparing this manuscript. These studies were supported, in part, by R01 HL074326, M01RR00047; and the Will Rogers Memorial Fund, Los Angeles, CA. BG was supported, in part, by The Chest Foundation, Northbrook, IL.
- Mills PR, Davies RJ, Devalia JL: Airway epithelial cells, cytokines, and pollutants. Am J Respir Crit Care Med 1999, 160:S38-S43.View ArticlePubMed
- Maestrelli P, Saetta M, Mapp CE, Fabbri LM: Remodeling in response to infection and injury. Airway inflammation and hypersecretion of mucus in smoking subjects with chronic obstructive pulmonary disease. Am J Respir Crit Care Med 2001, 164:S76-S80.View ArticlePubMed
- Spurzem JR, Rennard SI: Asthma and COPD. (Edited by: Barns P, Drazen J, Rennard S, Thompson N). Amsterdam 2002, 145.
- Hogg JC: Pathophysiology of airflow limitation in chronic obstructive pulmonary disease. Lancet 2004, 364:709–721.View ArticlePubMed
- van d V, Postma DS, Timens W, ten Hacken NH: Acute effects of cigarette smoke on inflammation and oxidative stress: a review. Thorax 2004, 59:713–721.View Article
- Simani AS, Inoue S, Hogg JC: Penetration of the respiratory epithelium of guinea pigs following exposure to cigarette smoke. Lab Invest 1974, 31:75–81.PubMed
- Jones JG, Minty BD, Lawler P, Hulands G, Crawley JC, Veall N: Increased alveolar epithelial permeability in cigarette smokers. Lancet 1980, 1:66–68.View ArticlePubMed
- Hulbert WC, Walker DC, Jackson A, Hogg JC: Airway permeability to horseradish peroxidase in guinea pigs: the repair phase after injury by cigarette smoke. Am Rev Respir Dis 1981, 123:320–326.PubMed
- Hogg JC, Chu F, Utokaparch S, Woods R, Elliott WM, Buzatu L, Cherniack RM, Rogers RM, Sciurba FC, Coxson HO, Pare PD: The nature of small-airway obstruction in chronic obstructive pulmonary disease. N Engl J Med 2004, 350:2645–2653.View ArticlePubMed
- Walker DC, MacKenzie A, Wiggs BR, Hulbert WC, Hogg JC: The structure of tight junctions in the tracheal epithelium may not correlate with permeability. Cell Tissue Res 1984, 235:607–613.View ArticlePubMed
- Burns AR, Hosford SP, Dunn LA, Walker DC, Hogg JC: Respiratory epithelial permeability after cigarette smoke exposure in guinea pigs. J Appl Physiol 1989, 66:2109–2116.PubMed
- Jeffery PK, Reid LM: The effect of tobacco smoke, with or without phenylmethyloxadiazole (PMO), on rat bronchial epithelium: a light and electron microscopic study. J Pathol 1981, 133:341–359.View ArticlePubMed
- Aoshiba K, Tamaoki J, Nagai A: Acute cigarette smoke exposure induces apoptosis of alveolar macrophages. Am J Physiol Lung Cell Mol Physiol 2001, 281:L1392-L1401.PubMed
- Hoshino Y, Mio T, Nagai S, Miki H, Ito I, Izumi T: Cytotoxic effects of cigarette smoke extract on an alveolar type II cell-derived cell line. Am J Physiol Lung Cell Mol Physiol 2001, 281:L509-L516.PubMed
- Carnevali S, Petruzzelli S, Longoni B, Vanacore R, Barale R, Cipollini M, Scatena F, Paggiaro P, Celi A, Giuntini C: Cigarette smoke extract induces oxidative stress and apoptosis in human lung fibroblasts. Am J Physiol Lung Cell Mol Physiol 2003, 284:L955-L963.View ArticlePubMed
- Liu L, Yuan Y, Li F, Liu H: Relationship between apoptosis and E-cadherin expression in bronchial epithelium of smoking mouse. J Huazhong Univ Sci Technolog Med Sci 2003, 23:216–218.View ArticlePubMed
- Bartalesi B, Cavarra E, Fineschi M, Lucattelli B, Martorana PA, Lungarelia G: Different lung responses to cigrette smoke in two strains of mice sensitive to oxidants. Eur Respir J 2005, 25:15–22.View ArticlePubMed
- Vayssier M, Banzet N, Francois D, Bellmann K, Polla BS: Tobacco smoke induces both apoptosis and necrosis in mammalian cells: differential effects of HSP70. Am J Physiol 1998, 275:L771-L779.PubMed
- Wickenden JA, Clarke MC, Rossi AG, Rahman I, Faux SP, Donaldson K, MacNee W: Cigarette smoke prevents apoptosis through inhibition of caspase activation and induces necrosis. Am J Respir Cell Mol Biol 2003, 29:562–570.View ArticlePubMed
- Liu X, Conner H, Kobayashi T, Kim H, Wen F, Abe S, Fang Q, Wang X, Hashimoto M, Bitterman P, Rennard SI: Cigarette smoke extract induces DNA damage but not apoptosis in human bronchial epithelial cells. Am J Respir Cell Mol Biol 2005, 33:121–129.View ArticlePubMed
- Orzaez D, de Jong AJ, Woltering EJ: A tomato homologue of the human protein PIRIN is induced during programmed cell death. Plant Mol Biol 2001, 46:459–468.View ArticlePubMed
- Hackett NR, Heguy A, Harvey BG, O'Connor TP, Luettich K, Flieder DB, Kaplan R, Crystal RG: Variability of antioxidant-related gene expression in the airway epithelium of cigarette smokers. Am J Respir Cell Mol Biol 2003, 29:331–343.View ArticlePubMed
- Heguy A, Harvey BG, O'Connor TP, Hackett NR, Crystal RG: Sampling-dependent up-regulation of gene expression in sequential samples of human airway epithelial cells. Mol Med 2003, 9:200–208.PubMed CentralView ArticlePubMed
- Kaplan R, Luettich K, Heguy A, Hackett NR, Harvey BG, Crystal RG: Monoallelic up-regulation of the imprinted H19 gene in airway epithelium of phenotypically normal cigarette smokers. Cancer Res 2003, 63:1475–1482.PubMed
- Karp PH, Moninger TO, Weber SP, Nesselhauf TS, Launspach JL, Zabner J, Welsh MJ: An in vitro model of differentiated human airway epithelia. Methods for establishing primary cultures. Methods Mol Biol 2002, 188:115–137.PubMed
- Wyatt TA, Schmidt SC, Rennard SI, Tuma DJ, Sisson JH: Acetaldehyde-stimulated PKC activity in airway epithelial cells treated with smoke extract from normal and smokeless cigarettes. Proc Soc Exp Biol Med 2000, 225:91–97.View ArticlePubMed
- Rosenfeld MA, Siegfried W, Yoshimura K, Yoneyama K, Fukayama M, Stier LE, Paakko PK, Gilardi P, Stratford-Perricaudet LD, Perricaudet M: Adenovirus-mediated transfer of a recombinant alpha 1-antitrypsin gene to the lung epithelium in vivo. Science 1991, 252:431–434.View ArticlePubMed
- Hersh J, Crystal RG, Bewig B: Modulation of gene expression after replication-deficient, recombinant adenovirus-mediated gene transfer by the product of a second adenovirus vector. Gene Ther 1995, 2:124–131.PubMed
- He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B: A simplified system for generating recombinant adenoviruses. Proc Natl Acad Sci USA 1998, 95:2509–2514.PubMed CentralView ArticlePubMed
- Ke Y, Reddel RR, Gerwin BI, Miyashita M, McMenamin M, Lechner JF, Harris CC: Human bronchial epithelial cells with integrated SV40 virus T antigen genes retain the ability to undergo squamous differentiation. Differentiation 1988, 38:60–66.View ArticlePubMed
- Hihara Y, Muramatsu M, Nakamura K, Sonoike K: A cyanobacterial gene encoding an ortholog of Pirin is induced under stress conditions. FEBS Lett 2004, 574:101–105.View ArticlePubMed
- Morrison D, Rahman I, Lannan S, MacNee W: Epithelial permeability, inflammation, and oxidant stress in the air spaces of smokers. Am J Respir Crit Care Med 1999, 159:473–479.View ArticlePubMed
- Wendler WM, Kremmer E, Forster R, Winnacker EL: Identification of pirin, a novel highly conserved nuclear protein. J Biol Chem 1997, 272:8482–8489.View ArticlePubMed
- Pang H, Bartlam M, Zeng Q, Miyatake H, Hisano T, Miki K, Wong LL, Gao GF, Rao Z: Crystal structure of human pirin: an iron-binding nuclear protein and transcription cofactor. J Biol Chem 2004, 279:1491–1498.View ArticlePubMed
- Sun W, Wu R, Last JA: Effects of exposure to environmental tobacco smoke on a human tracheobronchial epithelial cell line. Toxicology 1995, 100:163–174.View ArticlePubMed
- Dechend R, Hirano F, Lehmann K, Heissmeyer V, Ansieau S, Wulczyn FG, Scheidereit C, Leutz A: The Bcl-3 oncoprotein acts as a bridging factor between NF-kappaB/Rel and nuclear co-regulators. Oncogene 1999, 18:3316–3323.View ArticlePubMed
- Spira A, Beane J, Shah V, Liu G, Schembri F, Yang X, Palma J, Brody JS: Effects of cigarette smoke on the human airway epithelial cell transcriptome. Proc Natl Acad Sci USA 2004, 101:10143–10148.PubMed CentralView ArticlePubMed
- O'Sullivan MP, Tyner JW, Holtzman MJ: Apoptosis in the airways: another balancing act in the epithelial program. Am J Respir Cell Mol Biol 2003, 29:3–7.View ArticlePubMed
- Christine Pratt MA, Niu M, White D: Differential regulation of protein expression, growth and apoptosis by natural and synthetic retinoids. J Cell Biochem 2003, 90:692–708.View Article
- Jang TJ, Kang HJ, Kim JR, Yang CH: Non-steroidal anti-inflammatory drug activated gene (NAG-1) expression is closely related to death receptor-4 and -5 induction, which may explain sulindac sulfide induced gastric cancer cell apoptosis. Carcinogenesis 2004, 25:1853–1858.View ArticlePubMed
- Cobb LJ, Salih DA, Gonzalez I, Tripathi G, Carter EJ, Lovett F, Holding C, Pell JM: Partitioning of IGFBP-5 actions in myogenesis: IGF-independent anti-apoptotic function. J Cell Sci 2004, 117:1737–1746.View ArticlePubMed
- Piscione TD, Phan T, Rosenblum ND: BMP7 controls collecting tubule cell proliferation and apoptosis via Smad1-dependent and -independent pathways. Am J Physiol Renal Physiol 2001, 280:F19-F33.PubMed
- Duxbury MS, Ito H, Zinner MJ, Ashley SW, Whang EE: CEACAM6 gene silencing impairs anoikis resistance and in vivo metastatic ability of pancreatic adenocarcinoma cells. Oncogene 2004, 23:465–473.View ArticlePubMed
- Kanamori T, Takakura K, Mandai M, Kariya M, Fukuhara K, Sakaguchi M, Huh NH, Saito K, Sakurai T, Fujita J, Fujii S: Increased expression of calcium-binding protein S100 in human uterine smooth muscle tumours. Mol Hum Reprod 2004, 10:735–742.View ArticlePubMed
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.