Nicotinic receptors on rat alveolar macrophages dampen ATP-induced increase in cytosolic calcium concentration
© Mikulski et al. 2010
Received: 30 March 2010
Accepted: 29 September 2010
Published: 29 September 2010
Nicotinic acetylcholine receptors (nAChR) have been identified on a variety of cells of the immune system and are generally considered to trigger anti-inflammatory events. In the present study, we determine the nAChR inventory of rat alveolar macrophages (AM), and investigate the cellular events evoked by stimulation with nicotine.
Rat AM were isolated freshly by bronchoalveolar lavage. The expression of nAChR subunits was analyzed by RT-PCR, immunohistochemistry, and Western blotting. To evaluate function of nAChR subunits, electrophysiological recordings and measurements of intracellular calcium concentration ([Ca2+]i) were conducted.
Positive RT-PCR results were obtained for nAChR subunits α3, α5, α9, α10, β1, and β2, with most stable expression being noted for subunits α9, α10, β1, and β2. Notably, mRNA coding for subunit α7 which is proposed to convey the nicotinic anti-inflammatory response of macrophages from other sources than the lung was not detected. RT-PCR data were supported by immunohistochemistry on AM isolated by lavage, as well as in lung tissue sections and by Western blotting. Neither whole-cell patch clamp recordings nor measurements of [Ca2+]i revealed changes in membrane current in response to ACh and in [Ca2+]i in response to nicotine, respectively. However, nicotine (100 μM), given 2 min prior to ATP, significantly reduced the ATP-induced rise in [Ca2+]i by 30%. This effect was blocked by α-bungarotoxin and did not depend on the presence of extracellular calcium.
Rat AM are equipped with modulatory nAChR with properties distinct from ionotropic nAChR mediating synaptic transmission in the nervous system. Their stimulation with nicotine dampens ATP-induced Ca2+-release from intracellular stores. Thus, the present study identifies the first acute receptor-mediated nicotinic effect on AM with anti-inflammatory potential.
Alveolar macrophages (AM) hold a key position in initiating pulmonary inflammatory responses by secreting tumor necrosis factor α (TNFα) and several additional cytokines and chemokines. It has been demonstrated that TNFα production and release from peritoneal macrophages can be largely inhibited by neurally released ACh thereby attenuating systemic inflammatory responses. This physiological mechanism has been termed "cholinergic anti-inflammatory pathway" . Studies on monocyte-derived human macrophages and on nicotinic acetylcholine receptor (nAChR) deficient mouse strains revealed that the nAChR α7 subunit is essential for this anti-inflammatory pathway . It has been demonstrated that stimulation of mouse peritoneal macrophages with nicotine is associated with activation of the Jak2-STAT3 signaling pathway and with inhibition of the release of pro-inflammatory cytokines and chemokines . Several lines of evidence show that stimulation of the cholinergic anti-inflammatory pathway and application of nicotinic agonists can be beneficial in experimental endotoxemia and sepsis [1–3]. The α7 subunit is one of 9 different known ligand-binding α subunits (α1-α7 and α9-α10) that assemble to homo- or heteropentamers, partially with additional participation of β subunits, to form a functional nAChR. All these receptors are ligand-gated cation channels, and they are distinct from each other with respect to ligand affinity and to preference for mono- or divalent cations . There is growing evidence that neuronal-type ion channels are not formed by nAChR subunits in cells of the immune system [5–7].
In view of the natural occurrence of nAChR ligands in the alveolar compartment (e.g. choline) and of the clinical relevance of nicotine contained within cigarette smoke, the potential presence of a cholinergic anti-inflammatory pathway in the lung deserves high attention. Indeed, nAChR agonists reduce acid- and gram-negative sepsis-induced acute lung injury in mice and rats [8, 9] and tumour necrosis factor-α (TNF-α) release into the lung compartment after intrapulmonary delivery of LPS in mice . Here, we hypothesized that cholinergic anti-inflammation is operative through modulation of AM function. We established an inventory of nAChR subunit expression in rat AM by RT-PCR and immunohistochemistry. Whole-cell patch-clamp measurements were conducted to investigate whether classical, ion-conducting nAChR are operative in AM. The effect of nicotine upon macrophage stimulation with ATP, a "host tissue damage" or "danger signal" , was investigated by the method of real-time imaging for cytosolic Ca2+ responses. We demonstrate that there is a nicotinic anti-inflammatory pathway operative in rat AM. The receptor subtypes involved and intracellular signaling pathways, as identified so far, differ from that known from the nervous system. Potentially, this allows for selective pharmacological intervention and therapeutic use.
Alveolar macrophage isolation
Female Wistar rats (8-10 weeks old) were obtained from the local animal breeding facility (Institute of Physiology, Justus-Liebig-University, Giessen, Germany) and kept under conventional conditions. Wild type C57BL6N specific-pathogen free (SPF) mice were purchased from Charles River (Sulzfeld, Germany). Mice deficient for the α7 nAChR subunit were obtained from Jackson Laboratory (Bar Harbor, USA) and bred in SPF conditions by the local animal breeding facility using heterozygotes as breeders. Male and female mice were used throughout the study between 8 and 12 weeks of age. All animals were kept with free access to food and water. Animal care and animal experiments were performed following the current version of the German Law on the Protection of Animals as well as the NIH "principles of laboratory animal care".
Animals were killed by inhalation of an overdose of isoflurane (Abbott, Wiesbaden, Germany). For isolation of rat AM, the lung was carefully removed, cannulated via the trachea, and bronchoalveolar lavage (BAL) was performed using 10 × 5 ml ice-cold PBS containing (in mM): KCl 2.68, KH2PO4 1.47, NaCl 136.89, Na2HPO4 8.10 (pH 7.3) (PAA, Pasching, Austria). Mouse AM were isolated according to previously described protocols . The lavage fluid was centrifuged at 400 × g for 5 min at 4°C, and the pellet was resuspended in PBS or DMEM/F12 GlutaMax-I medium (Invitrogen, Karlsruhe, Germany). BAL cells were monitored by microscopy, and preparations containing erythrocytes were discarded. Isolated macrophages were used for subsequent analysis by RT-PCR, immunocytochemistry, Western blotting, electrophysiological recordings and measurements of intracellular calcium concentration ([Ca2+]i).
Rat primer sequences used in the study.
Mouse primer sequences used in the study.
Differences were noted for two methods used to prepare cDNA. Successful detection of rat α9 subunit mRNA required reverse transcription with Superscript II system. Amplification of rat α9 subunit mRNA from BAL cDNA generated with iScript enzyme was not successful. This may be due to different priming strategies (oligo(dT) and blend of oligo(dT) + random hexamer primers, respectively) or to reduced RNAse H activity in SuperScriptII enzyme, which enables more efficient cDNA synthesis .
Lavaged rat cells (n = 10 animals) were plated on polystyrene 8-well culture slides (BD Biosciences, Erembodegem, Belgium) in DMEM/F12 supplemented with penicillin (100 U/ml) and streptomycin (0.1 mg/ml). Cells were allowed to attach for 2 h, and then fixed in acetone (-20°C, 10 min) or isopropanol (+4°C, 10 min) and air-dried for 1 h.
Antibodies used in the study.
Synthetic peptide (aa 466-474 of human sequence)a
Synthetic peptide (620-627, human)a
Synthetic peptide (460-468, human)a
Synthetic peptide (493-502, human)a
Native and denatured α7 subunit (380-400, chicken) and denatured α7 subunit from rat
Mouse, monoclonal, clone mAb 306
Synthetic peptides (81-97 and 115-128, rat)
Synthetic peptide (404-418, rat)a
Synthetic peptide (493-502, human)a
Synthetic peptide (450-458, human)a
Synthetic peptide (490-498, human)a
Rat spleen cells
Mouse, monoclonal, clone ED1
Synthetic phospho-peptide residues surrounding Tyr705 of mouse Stat3
Rabbit, monoclonal, clone D3A7
Synthetic phospho-peptide residues surrounding Ser727 of mouse Stat3
Synthetic peptide corresponding to the sequence of mouse Stat3
SDS-PAGE and immunoblotting
nAChR α7 and α10 subunits detection
Snap-frozen rat BAL cells, rat brain and skin samples were homogenized and boiled in Laemmli's sample buffer  containing Complete® protease inhibitor cocktail (Roche, Mannheim, Germany). SDS-PAGE was carried out using 15% polyacrylamide gels according to Laemmli et al. . Samples (5 × 104 cells) and Rainbow™ colored molecular mass markers (Amersham Pharmacia Biotech, Freiburg, Germany) were separated on the same gel. Proteins were transferred electrophoretically onto Immobilon™-P PVDF membranes (Millipore, Bedford, USA) using a blotting buffer consisting of 25 mM Tris, 192 mM glycine, 20% methanol and 0.05% SDS. Membranes were pre-incubated with PBS containing 10% Rotiblock (Roth, Karlsruhe, Germany) solution for 1 h. Primary mouse-anti-nAChR α7 (1:1000, Sigma-Aldrich, Taufkirchen, Germany) antibodies were diluted in blocking solution and incubated with membranes overnight at 4°C. For the detection of α10 subunits, membranes were pre-incubated with PBS containing 5% non-fat milk powder (Roth) and guinea pig-anti-nAChR α10 (1:4000, ) antibodies were used. Blots were washed in PBS, 0.05% Tween 20 and bound primary antibodies were detected by horseradish peroxidase-conjugated immunoglobulins (DAKO, Hamburg, Germany) in PBS, 2% low fat milk, 0.05% Tween 20 (TPBS). Secondary antisera were rabbit anti-mouse IgG (1:5000 in TPBS + 1% normal rat serum) and rabbit-anti-guinea pig IgG (1:5000 in TPBS + 2% low fat milk). Peroxidase activity was visualized by SuperSignal® West Pico Chemiluminescent Substrate (Pierce, Rockford, IL, USA) using the Kodak Scientific Imaging Film X-OMAT™ LS (Eastman Kodak, Rochester, NY, USA). Gels and blots were documented and densitometrically analyzed using a digital gel documentation system (Biozym, Hessisch Oldendorf, Germany).
Lavaged rat cells were plated on a 24 well plate (Becton Dickinson, USA) at 3.5 × 105 cells/well in RPMI 1640 medium supplemented with L-glutamine, penicillin (100 U/ml) and streptomycin (0.1 mg/ml). Cells were allowed to attach for 2 h and subsequently were stimulated with nicotine (Sigma-Aldrich) at 10-4 M, 10-5 M, and 10-6 M or GM-CSF (R&D Systems, Minneapolis, MN) at 100 ng/ml for indicated time intervals. Cells were washed twice with cold PBS and lysed with lysis buffer containing 20 mM Tris (pH 7.5), 150 mM NaCl, 1 mM EDTA (pH 8.0), 1 mM EGTA (pH 8.0), 0.5% NP-40, 2 mM sodium orthovanadate (pH 10.0), and Complete® protease inhibitor cocktail (Roche). The lysates were kept on ice for 30 min, followed by centrifugation for 15 min at 13,000 rpm at 4°C, and subsequent protein concentration measurement was assessed by Bradford Assay as suggested by the manufacturer (Bio-Rad). Proteins were loaded on a gel in a total amount of 10 μg, separated by electrophoresis on 10% SDS-PAGE, and transferred to polyvinylidene difluoride membranes (Amersham GE Healthcare, Little Chalfont, Buckinghamshire, UK). Membranes were incubated in blocking buffer (5% non-fat milk in PBS, 0.05% Tween 20) at room temperature for 1 h, and then overnight at 4°C with primary antibodies recognizing total and phosphorylated STAT3 (1:1000, Cell Signaling Technology, Beverly, MA). Blots were washed three times for 15 min, and incubated with horseradish peroxidase-conjugated anti-rabbit IgG (1:3500, Pierce, Rockford, IL). Enhanced chemiluminescence system was used to visualize immune complexes (Amersham GE Healthcare, Little Chalfont, Buckinghamshire, UK).
For whole cell patch-clamp recordings, 200 μl cell suspension obtained from rat BAL was placed in recording dishes (Nunc, Roskilde, Denmark), incubated for 1-3 h at 37°C and 5% CO2 in DMEM/F12 medium to allow the cells to attach. PC12 cells (rat adrenal pheochromocytoma cells) were obtained from German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany) and maintained at 37°C and 5% CO2 in RPMI 1640 medium supplemented with 10% horse serum (PAA), 5% FCS, 2 mM L-glutamine, penicillin (100 U/ml), streptomycin (0.1 mg/ml) and used as described above.
For recordings, the medium was carefully removed, cells were washed 2-3 times, covered with bath solution containing (in mM): NaCl 120, KCl 5.4, CaCl2 2, MgCl2 1, Hepes 10, D-glucose 25, pH 7.5, and the dish was placed under a microscope (Axiovert, Göttingen, Germany).
Borosilicate glass capillaries (Hilgenberg, Malsfeld, Germany) with an outer diameter of 1.6 mm were pulled to recording pipettes by a vertical puller (Narishige, Tokyo, Japan). The tips of the pipettes were fire-polished using a microforge (List-Medical, Darmstadt, Germany) and had resistances between 5-10 MΩ when filled with the pipette solution containing (in mM): KCl 120, CaCl2 1, MgCl2 2, Hepes 10, EGTA 11, D-glucose 20, pH 7.3. The junction potential under these conditions (bath and pipette solution) was 3.4 mV although the membrane voltage was not corrected with respect to this junction potential.
The whole cell configuration was mainly obtained by suction and in some cases voltage pulse was additionally applied. Transmembrane currents were recorded at holding potentials of -60 mV in the absence of, as well as after ACh application into the bath. The agonist was applied via a pipette to the bath to reach a final concentration of 100 μM. In some experiments, a VM-4 micro-perfusion system was used for drug compound application (ALA Scientific Instruments, Westbury, USA). The measured signals were amplified by an EPC-9 patch-clamp amplifier (HEKA, Lambrecht/Pfalz, Germany), which was connected via an ITC-16 interface to a personal computer. For continuous recordings, the signals were filtered with 300 Hz and acquired at 3 kHz using the Pulse 8.77 software (HEKA). Current/voltage relationships signals were filtered with 3.33 kHz and acquired at 10 kHz. Data ware analyzed and prepared using PulseFit (HEKA) and Igor (Wavemetrics, Lake Oswego, USA). All recordings were performed at room temperature.
Intracellular calcium concentration measurements
Recordings of [Ca2+]i were performed after 3-8 h in primary culture (n = 3 animals and 10-12 coverslips for each experimental setup). Measurements were done in Hepes buffer containing (in mM): KCl 5.6, NaCl 136.4, MgCl2 1, CaCl2 2.2, D-glucose 11, Hepes 10. In some experiments, CaCl2 was omitted from the buffer composition. Cells were loaded for 30 min with 3.3 μM fura-2 AM (Invitrogen) and washed 3 × 10 min. Fura-2 was excited at 340 and 380 nm wavelengths (λ), and fluorescence was collected at λ > 420 nm. The fluorescence intensity ratio of 340/380 nm was recorded. Cells were exposed to nicotine (10-6-10-4 M, Sigma-Aldrich) or epibatidine (10-6 M, Sigma-Aldrich). Controls were performed with vehicle treatment. Two min after administration of nicotine or epibatidine, cells were stimulated with ATP (10-4 M, Sigma-Aldrich). In some experiments cells were exposed to nicotine in the presence of the nAChR α7 and α9/α10 blocker α-bungarotoxin (10-7 M, Sigma-Aldrich). Cells that did not respond to ATP by at least 5% change in [Ca2+]i were excluded from further analysis. Viability of the cells was monitored after measurements with Trypan Blue exclusion. Ratio values were normalized to 100% at the beginning of recording. Curves were plotted from recordings done in preparations from n = 3 animals. Data are shown as mean ± SEM.
Data in the figures and text are expressed as mean ± SEM. Non-parametric rank based Kruskal-Wallis test was used to compare multiple groups, and if significant differences were detected, it was followed by Mann-Whitney test to compare between two experimental groups. Tests were performed using SPSS software (SPSS software, Munich, Germany). P ≤ 0.05 was considered significant and P ≤ 0.01 as highly significant.
Rat alveolar macrophages constitutively express nAChR subunits α9, α10 and β2, but not α7
Acetylcholine has no effect on AM cell membrane conductivity
For further characterization of AM responses, I/V curves were recorded by applying voltage steps of 20 mV from -100 to 100 mV, starting from a holding potential of -60 mV. I/V-relationships were also recorded in the absence (Fig. 5F) and presence of ACh (Fig. 5E). Again, no changes of IM were evoked by ACh application.
ATP-triggered increase in calcium derives from intracellular stores
Nicotine modulates ATP-induced rise in intracellular [Ca2+]
In a separate set of experiments, we tested if the effect of nicotine can be blocked with the α1, α7 and α9/α9α10 nAChR antagonist α-bungarotoxin. This drug alone (10-7 M) had no effect on ATP-induced calcium increase, when compared to vehicle control, but it abrogated the effects of nicotine (P ≤ 0.001). The transient rise in [Ca2+]i in cells treated with α-bungarotoxin together with nicotine was slightly increased compared to vehicle-treated cells (P ≤ 0.025) (Fig. 7B).
Nicotinic modulation is not dependent on extracellular calcium
Next we tested if the nicotine-mediated effect upon the ATP-induced [Ca2+]i rise is depended on extracellular calcium. Rat cells treated with nicotine (10-4 M) 2 min before the ATP stimulus showed a decreased amplitude of the ATP-induced rise in [Ca2+]i. This was not affected by the absence of Ca2+ in the external bath solution (Fig. 7B). The number of cells reacting to ATP was reduced when Ca2+ was omitted in the external solution (18% for vehicle and 16% for nicotine treated cells) compared to Ca2+-supplemented medium (42% for vehicle and 39% for nicotine treated cells).
Nicotine does not induce STAT-3 phosphorylation
This study is the first to demonstrate acute receptor-dependent, modulatory effects of nicotine on AM. The nAChR involved in this process differ from subtypes reported previously to be involved in "cholinergic anti-inflammatory pathways" outside the lung. Although the effects of nicotine are receptor mediated, these receptors do not form a classical ion channel known from neuronal cells.
Importantly, we detected neither mRNA nor protein of α7 nAChR in AM in contrast to easily detectable α7 subunit mRNA in sensory neurons, brain and in the whole lung homogenate. This is consistent with reported data on the lack of the expression of α7 nAChR in the murine AM cell line MH-S  and our previous work on expression of nAChR in freshly isolated murine AM . In contrast, binding of the polyclonal nAChR α7 antibody H-320 to murine AM has been reported by Su et al. [8, 9] and was also noted by our group in a previous study in the absence of α7 subunit mRNA detection . This antibody, however, produces identical staining in immunohistochemistry and western blotting of the mouse brain, clearly demonstrating lack of specificity at least in the nervous system , so that these findings have to be considered with caution unless corresponding controls on mouse lungs from α7 nAChR-/- mice have been successfully performed.
Still, there might be species differences and plasticity in receptor expression under pathological conditions, since a low level of basal expression of α7 nAChR subunit mRNA in AM isolated from healthy volunteers and an increase in AM isolated from smokers has been reported . Also, α7 nAChR are essential for systemic cholinergic anti-inflammation since the beneficial effects of nicotine in endotoxemia are abrogated in α7 subunit gene-deficient mice . Accordingly, two potent α7 nAChR agonists, GTS-21 and PNU-282987 [22, 23], inhibit LPS-induced TNFα release and reduce acid-induced acute lung injury, respectively, in the mouse lung [8, 10]. Their potency on the most prevalent nAChR subunits identified in our present study on AM, i.e. α9 and α10 nAChR that generally share many pharmacological properties with α7 nAChR , yet has not been determined. Without doubt, however, α7 nAChR is expressed in the lung as demonstrated by RT-PCR in this and previous studies [25, 26]. Functional data show increases in acid-induced excess lung water and vascular permeability in α7 nAChR deficient mice . Endothelial cells may account for this effect . However, since all α7 nAChR antibodies tested so far produce immunohistochemical labeling also in organs taken from α7 nAChR deficient mice [20, 28], immunohistochemistry alone cannot decipher the cell-type specific α7-subunit distribution in the lung, and this issue remains to be solved.
Instead of α7 we observed expression of nAChR subunits α9, α10, β1, and β2, and to a variable extent α2, α3, α5, in rat AM. Mouse AM expressed nAChR subunits α9, α10, β2, and β4. To form classical, ion-conducting nAChR, α subunits combine as heteropentamers with β subunits or build α heteropentamers of α9α10 and homopentamers of α7 and α9 (for review, see ). The subunits detected in AM in the present study would allow combining the following nAChR pentamers: α3β2, α3α5β2, α9α10, and α9 as homopentamer. Since there is a constant expression of subunits α9 and α10 in AM, this combination as homo- or heteropentamer seems to be most likely, if pentamer formation occurs at all.
These subunits have been best characterized in the inner ear, where they form Ca2+-permeable ion channels involved in efferent modulation of hair cell function [30, 31]. Our whole-cell patch clamp recordings and [Ca2+]i measurements in rat AM, however, neither revealed changes in membrane current in response to ACh nor in [Ca2+]i in response to nicotine. Similarly, a subpopulation of human T-lymphocytes expresses α9 and α10 nAChR subunits but fails to show transmembrane currents triggered by ACh , and nicotine does not cause alteration of [Ca2+]i in the rat AM cell line NR8383  and in rat intravascular mononuclear leukocytes obtained from isogenic kidney transplants . Thus, α9α10 nAChR subunits apparently do not form classical ionotropic receptors in cells of the immune system. Still, α9α10 nAChR subunits confer intracellular effects as our data demonstrate an acute α-bungarotoxin sensitive modulatory effect of nicotine upon ATP-induced calcium release from intracellular stores. In general, although to a much smaller extent than in rat cells, this effect was also present in macrophages isolated from C57BL6N and α7 nAChR subunit deficient mice, demonstrating its independency from the α7 nAChR subunit. Similarly, we recently identified a methyllycaconitine sensitive modulatory effect of nicotine upon ATP-induced rise in [Ca2+]i in rat mononuclear leukocytes obtained by vascular perfusion of isogenic kidney transplants . In line with this observation, α9 subunit containing nAChR in outer hair cells of the inner ear do not exclusively assemble into ionotropic receptors, but form metabotropic receptors as well. Here, ACh also reduces ATP-induced rise in [Ca2+]i at a concentration that alone is insufficient to impact [Ca2+]i, and again this effect is α-bungarotoxin sensitive .
Atypical, non-ionotropic effects have also been reported for the nAChR α7 subunit. In T cells, this subunit fails to form a ligand-gated Ca2+ channel but interacts with CD3ζ to modulate TCR/CD3 function . Notably, α7 subunits in this complex exhibit a different agonist/antagonists profile than neuronal ionotropic α7 nAChR. Methyllycaconitine and α-bungarotoxin, both potent inhibitors of ionotropic α7 nAChR, indeed are strong agonists at T cells expressing nAChR α7 subunits . Correspondingly, epibatidine, a highly potent agonist at ionotropic nAChR, failed to mimic the nicotine effect in our present experiments on rat AM.
In contrast to the well-characterized channel properties of nAChR, the mechanisms of atypical nAChR signaling are currently only poorly understood. In peritoneal macrophages, coupling of α7 nAChR to the Jak2-STAT3 signaling pathway resulting in STAT3 phosphorylation has been reported  which we could not observe in rat AM predominantly expressing α9 and α10 subunits. Membrane bound nAChR subunits have been demonstrated to interact with and to modulate signaling by β-arrestin , phosphatidyl-inositol-3-kinase , CD3ζ , and purinergic P2X-receptors [36, 37]. The latter are involved in ATP-induced increase in [Ca2+]i by extracellular influx in human AM, since initial Ca2+ transients are reduced by 40% in Ca2+-free medium . In our present study of rat AM, however, the ATP-induced initial increase in [Ca2+]i and the modulatory effect of nicotine persisted in Ca2+-free solution, demonstrating interference of atypical nAChR with P2Y-receptor mediated Ca2+-release from intracellular stores. In support, we observed expression of P2Y purinergic receptors on AM, among them P2Y2 that mediates Ca2+-release from the endoplasmatic reticulum in mouse macrophages .
Extracellular ATP is well recognized as a "danger" or "host tissue damage" signal and is mostly regarded to promote inflammation [39, 40]. In human AM, it couples to [Ca2+]i increases and stimulates IL-1β and IL-6 release albeit suppressing TNFα production . In the rat AM cell line NR8383, ATP induces P2Y2- and Ca2+-dependent increase in CCL2 synthesis and release . The CCL2-CCR2 axis is a crucial regulator of inflammatory cell influx into the murine lung [42, 43]. Hence, the presently observed nicotinic attenuation of ATP-induced rise in [Ca2+]i can be considered as an anti-inflammatory mechanism triggered by atypical nAChR.
Rat AM are equipped with modulatory nAChR with properties distinct from ionotropic nAChR mediating synaptic transmission in the nervous system. Their stimulation with nicotine dampens ATP-induced Ca2+-release from intracellular stores. Thus, the present study identifies the first acute receptor-mediated but atypical nicotinic effect on AM with anti-inflammatory potential.
The authors wish to thank Ms. Sigrid Wilker and Mr. Martin Bodenbenner for excellent technical assistance, Dr. Gabriela Krasteva for help with the confocal microscopy and Ms. Karola Michael for help with the art work. This study was supported by the DFG (Excellence Cluster "Cardiopulmonary System" and IntGK 1062) and a grant of the University Medical Center Giessen and Marburg.
- Borovikova LV, Ivanova S, Zhang M, Yang H, Botchkina GI, Watkins LR, Wang H, Abumrad N, Eaton JW, Tracey KJ: Vagus nerve stimulation attenuates the systemic inflammatory response to endotoxin. Nature 2000,405(6785):458–462.View ArticlePubMed
- Wang H, Yu M, Ochani M, Amella CA, Tanovic M, Susarla S, Li JH, Yang H, Ulloa L, Al-Abed Y, et al.: Nicotinic acetylcholine receptor alpha7 subunit is an essential regulator of inflammation. Nature 2003,421(6921):384–388.View ArticlePubMed
- de Jonge WJ, van der Zanden EP, The FO, Bijlsma MF, van Westerloo DJ, Bennink RJ, Berthoud HR, Uematsu S, Akira S, van den Wijngaard RM, et al.: Stimulation of the vagus nerve attenuates macrophage activation by activating the Jak2-STAT3 signaling pathway. Nat Immunol 2005,6(8):844–851.View ArticlePubMed
- Lukas RJ, Changeux JP, Le Novere N, Albuquerque EX, Balfour DJ, Berg DK, Bertrand D, Chiappinelli VA, Clarke PB, Collins AC, et al.: International Union of Pharmacology. XX. Current status of the nomenclature for nicotinic acetylcholine receptors and their subunits. Pharmacol Rev 1999,51(2):397–401.PubMed
- Peng H, Ferris RL, Matthews T, Hiel H, Lopez-Albaitero A, Lustig LR: Characterization of the human nicotinic acetylcholine receptor subunit alpha (alpha) 9 (CHRNA9) and alpha (alpha) 10 (CHRNA10) in lymphocytes. Life Sci 2004,76(3):263–280.View ArticlePubMed
- Razani-Boroujerdi S, Boyd RT, Davila-Garcia MI, Nandi JS, Mishra NC, Singh SP, Pena-Philippides JC, Langley R, Sopori ML: T cells express alpha7-nicotinic acetylcholine receptor subunits that require a functional TCR and leukocyte-specific protein tyrosine kinase for nicotine-induced Ca2+ response. J Immunol 2007,179(5):2889–2898.PubMed
- Hecker A, Mikulski Z, Lips KS, Pfeil U, Zakrzewicz A, Wilker S, Hartmann P, Padberg W, Wessler I, Kummer W, et al.: Pivotal Advance: Up-regulation of acetylcholine synthesis and paracrine cholinergic signaling in intravascular transplant leukocytes during rejection of rat renal allografts. J Leukoc Biol 2009,86(1):13–22.View ArticlePubMed
- Su X, Lee JW, Matthay ZA, Mednick G, Uchida T, Fang X, Gupta N, Matthay MA: Activation of the alpha7 nAChR reduces acid-induced acute lung injury in mice and rats. Am J Respir Cell Mol Biol 2007,37(2):186–192.View ArticlePubMed
- Su X, Matthay MA, Malik AB: Requisite role of the cholinergic alpha7 nicotinic acetylcholine receptor pathway in suppressing Gram-negative sepsis-induced acute lung inflammatory injury. J Immunol 2010,184(1):401–410.View ArticlePubMed
- Giebelen IA, van Westerloo DJ, LaRosa GJ, de Vos AF, van der Poll T: Local stimulation of alpha7 cholinergic receptors inhibits LPS-induced TNF-alpha release in the mouse lung. Shock 2007,28(6):700–703.PubMed
- del Rey A, Renigunta V, Dalpke AH, Leipziger J, Matos JE, Robaye B, Zuzarte M, Kavelaars A, Hanley PJ: Knock-out mice reveal the contributions of P2Y and P2X receptors to nucleotide-induced Ca2+ signaling in macrophages. J Biol Chem 2006,281(46):35147–35155.View ArticlePubMed
- Mikulski Z, Zaslona Z, Cakarova L, Hartmann P, Wilhelm J, Tecott LH, Lohmeyer J, Kummer W: Serotonin activates murine alveolar macrophages through 5-HT2C receptors. Am J Physiol Lung Cell Mol Physiol 2010,299(2):L272–80.View ArticlePubMed
- Gerard GF, Fox DK, Nathan M, D'Alessio JM: Reverse transcriptase. The use of cloned Moloney murine leukemia virus reverse transcriptase to synthesize DNA from RNA. Mol Biotechnol 1997,8(1):61–77.View ArticlePubMed
- Krasteva G, Pfeil U, Drab M, Kummer W, Konig P: Caveolin-1 and -2 in airway epithelium: expression and in situ association as detected by FRET-CLSM. Respir Res 2006, 7:108.View ArticlePubMed
- Dijkstra CD, Dopp EA, Joling P, Kraal G: The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3. Immunology 1985,54(3):589–599.PubMed
- Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970,227(5259):680–685.View ArticlePubMed
- Lips KS, Pfeil U, Kummer W: Coexpression of alpha 9 and alpha 10 nicotinic acetylcholine receptors in rat dorsal root ganglion neurons. Neuroscience 2002,115(1):1–5.View ArticlePubMed
- Matsunaga K, Klein TW, Friedman H, Yamamoto Y: Involvement of nicotinic acetylcholine receptors in suppression of antimicrobial activity and cytokine responses of alveolar macrophages to Legionella pneumophila infection by nicotine. J Immunol 2001,167(11):6518–6524.PubMed
- Galvis G, Lips KS, Kummer W: Expression of nicotinic acetylcholine receptors on murine alveolar macrophages. J Mol Neurosci 2006,30(1–2):107–108.View ArticlePubMed
- Herber DL, Severance EG, Cuevas J, Morgan D, Gordon MN: Biochemical and histochemical evidence of nonspecific binding of alpha7nAChR antibodies to mouse brain tissue. J Histochem Cytochem 2004,52(10):1367–1376.View ArticlePubMed
- Prasse A, Stahl M, Schulz G, Kayser G, Wang L, Ask K, Yalcintepe J, Kirschbaum A, Bargagli E, Zissel G, et al.: Essential role of osteopontin in smoking-related interstitial lung diseases. Am J Pathol 2009,174(5):1683–1691.View ArticlePubMed
- Hajos M, Hurst RS, Hoffmann WE, Krause M, Wall TM, Higdon NR, Groppi VE: The selective alpha7 nicotinic acetylcholine receptor agonist PNU-282987 [N-[(3R)-1-Azabicyclo[2.2.2]oct-3-yl]-4-chlorobenzamide hydrochloride] enhances GABAergic synaptic activity in brain slices and restores auditory gating deficits in anesthetized rats. J Pharmacol Exp Ther 2005,312(3):1213–1222.View ArticlePubMed
- Meyer EM, Tay ET, Papke RL, Meyers C, Huang GL, de Fiebre CM: 3-[2,4-Dimethoxybenzylidene]anabaseine (DMXB) selectively activates rat alpha7 receptors and improves memory-related behaviors in a mecamylamine-sensitive manner. Brain Res 1997,768(1–2):49–56.View ArticlePubMed
- Baker ER, Zwart R, Sher E, Millar NS: Pharmacological properties of alpha 9 alpha 10 nicotinic acetylcholine receptors revealed by heterologous expression of subunit chimeras. Mol Pharmacol 2004,65(2):453–460.View ArticlePubMed
- Reynolds PR, Hoidal JR: Temporal-spatial expression and transcriptional regulation of alpha7 nicotinic acetylcholine receptor by thyroid transcription factor-1 and early growth response factor-1 during murine lung development. J Biol Chem 2005,280(37):32548–32554.View ArticlePubMed
- Grau V, Wilker S, Hartmann P, Lips KS, Grando SA, Padberg W, Fehrenbach H, Kummer W: Administration of keratinocyte growth factor (KGF) modulates the pulmonary expression of nicotinic acetylcholine receptor subunits alpha7, alpha9 and alpha10. Life Sci 2007,80(24–25):2290–2293.View ArticlePubMed
- Heeschen C, Weis M, Aicher A, Dimmeler S, Cooke JP: A novel angiogenic pathway mediated by non-neuronal nicotinic acetylcholine receptors. J Clin Invest 2002,110(4):527–536.PubMed
- Moser N, Mechawar N, Jones I, Gochberg-Sarver A, Orr-Urtreger A, Plomann M, Salas R, Molles B, Marubio L, Roth U, et al.: Evaluating the suitability of nicotinic acetylcholine receptor antibodies for standard immunodetection procedures. J Neurochem 2007,102(2):479–492.View ArticlePubMed
- Millar NS, Gotti C: Diversity of vertebrate nicotinic acetylcholine receptors. Neuropharmacology 2009,56(1):237–246.View ArticlePubMed
- Vetter DE, Liberman MC, Mann J, Barhanin J, Boulter J, Brown MC, Saffiote-Kolman J, Heinemann SF, Elgoyhen AB: Role of alpha9 nicotinic ACh receptor subunits in the development and function of cochlear efferent innervation. Neuron 1999,23(1):93–103.View ArticlePubMed
- Vetter DE, Katz E, Maison SF, Taranda J, Turcan S, Ballestero J, Liberman MC, Elgoyhen AB, Boulter J: The alpha10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system. Proc Natl Acad Sci USA 2007,104(51):20594–20599.View ArticlePubMed
- Zhang GH, Helmke RJ, Mork AC, Martinez JR: Regulation of cytosolic free Ca2+ in cultured rat alveolar macrophages (NR8383). J Leukoc Biol 1997,62(3):341–348.PubMed
- Wikstrom MA, Lawoko G, Heilbronn E: Cholinergic modulation of extracellular ATP-induced cytoplasmic calcium concentrations in cochlear outer hair cells. J Physiol Paris 1998,92(5–6):345–349.View ArticlePubMed
- Dasgupta P, Rastogi S, Pillai S, Ordonez-Ercan D, Morris M, Haura E, Chellappan S: Nicotine induces cell proliferation by beta-arrestin-mediated activation of Src and Rb-Raf-1 pathways. J Clin Invest 2006,116(8):2208–2217.View ArticlePubMed
- Blanchet MR, Israel-Assayag E, Daleau P, Beaulieu MJ, Cormier Y: Dimethyphenylpiperazinium, a nicotinic receptor agonist, downregulates inflammation in monocytes/macrophages through PI3K and PLC chronic activation. Am J Physiol Lung Cell Mol Physiol 2006,291(4):L757–763.View ArticlePubMed
- Khakh BS, Zhou X, Sydes J, Galligan JJ, Lester HA: State-dependent cross-inhibition between transmitter-gated cation channels. Nature 2000,406(6794):405–410.View ArticlePubMed
- Khakh BS, Fisher JA, Nashmi R, Bowser DN, Lester HA: An angstrom scale interaction between plasma membrane ATP-gated P2X2 and alpha4beta2 nicotinic channels measured with fluorescence resonance energy transfer and total internal reflection fluorescence microscopy. J Neurosci 2005,25(29):6911–6920.View ArticlePubMed
- Myrtek D, Muller T, Geyer V, Derr N, Ferrari D, Zissel G, Durk T, Sorichter S, Luttmann W, Kuepper M, et al.: Activation of human alveolar macrophages via P2 receptors: coupling to intracellular Ca2+ increases and cytokine secretion. J Immunol 2008,181(3):2181–2188.PubMed
- Gavala ML, Pfeiffer ZA, Bertics PJ: The nucleotide receptor P2RX7 mediates ATP-induced CREB activation in human and murine monocytic cells. J Leukoc Biol 2008,84(4):1159–1171.View ArticlePubMed
- Idzko M, Hammad H, van Nimwegen M, Kool M, Willart MA, Muskens F, Hoogsteden HC, Luttmann W, Ferrari D, Di Virgilio F, et al.: Extracellular ATP triggers and maintains asthmatic airway inflammation by activating dendritic cells. Nat Med 2007,13(8):913–919.View ArticlePubMed
- Stokes L, Surprenant A: Purinergic P2Y2 receptors induce increased MCP-1/CCL2 synthesis and release from rat alveolar and peritoneal macrophages. J Immunol 2007,179(9):6016–6023.PubMed
- Maus UA, Koay MA, Delbeck T, Mack M, Ermert M, Ermert L, Blackwell TS, Christman JW, Schlondorff D, Seeger W, et al.: Role of resident alveolar macrophages in leukocyte traffic into the alveolar air space of intact mice. Am J Physiol Lung Cell Mol Physiol 2002,282(6):L1245–1252.PubMed
- Maus UA, Waelsch K, Kuziel WA, Delbeck T, Mack M, Blackwell TS, Christman JW, Schlondorff D, Seeger W, Lohmeyer J: Monocytes are potent facilitators of alveolar neutrophil emigration during lung inflammation: role of the CCL2-CCR2 axis. J Immunol 2003,170(6):3273–3278.PubMed
- Kurzen H, Berger H, Jager C, Hartschuh W, Naher H, Gratchev A, Goerdt S, Deichmann M: Phenotypical and molecular profiling of the extraneuronal cholinergic system of the skin. J Invest Dermatol 2004,123(5):937–949.View ArticlePubMed
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